The Influence of Zoledronic Acid on the Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Tibial Fracture in Rats via Phosphatidylinositol-3-Kinase/AKT Pathway-Reference-Cited by-全球学者库

The Influence of Zoledronic Acid on the Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Tibial Fracture in Rats via Phosphatidylinositol-3-Kinase/AKT Pathway

Published:2023-02-01 Issue:2 Volume:13 Page:325-333
ISSN:2157-9083
Container-title:Journal of Biomaterials and Tissue Engineering
language:en
Short-container-title:j biomater tissue eng

Author:

Liu JianZhou¹,Wang Hao¹,Shang GuoJun²,Lv XiangYang¹,Xu ZhenWei¹,Xiong FuJun¹

Affiliation:

1. Department of Orthopaedics, Xi’an International Medical Center Hospital, Xi’an City, Shaanxi Province, 710100, China

2. Department of Orthopaedics, The Second Hospital of Shanxi Medical University, TaiYuan City, ShanXi Province, 030053, China

Abstract

Fracture healing is an extremely complex physiological process, involving a sequence of crucial mechanisms. Whether zoledronic acid (ZA) affects proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and tibial fractures still remains unknown. We performed construction of the tibial fracture model in rats and analyzed the impact of ZA and ZA+LY294002 on fracture healing in rats. Test of the influence of ZA and ZA+LY294002 on crucial osteogenic genes alkaline phosphatase (ALP), RUNX2, OCN and OPN was performed. Examination of the influence of suppressing the PI3K/AKT pathway on the proliferation with bone differentiation of ZA. Results showed ZA distinctly accelerated the proliferation and ALP activity of BMSC cells, BMP2, RUNX2, OCN, OPN and the activation of the PI3K/AKT pathway. Repression of PI3K/AKT pathway suppressed the proliferation and osteogenic differentiation action of ZA. ZA boosted tibial fracture healing in rats via activating the PI3K/AKT pathway. ZA facilitates the proliferation with osteogenic differentiation of BMSC cells and tibial fracture healing in rats via activating the PI3K/AKT pathway.

Publisher

American Scientific Publishers

Subject

Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology

Reference23 articles.

1. Fibrinogen Induces RUNX2 Activity and Osteogenic Development from Human Pluripotent Stem Cells

2. Vertebral Bone Marrow-Derived Mesenchymal Stromal Cells from Osteoporotic and Healthy Patients Possess Similar Differentiation Properties In Vitro

3. Effects of once-yearly zoledronic acid on bone density and incident vertebral fractures in nonmetastatic castration-sensitive prostate cancer patients with osteoporosis

4. Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro

5. New agents in the Treatment of Myeloma Bone Disease