Affiliation:
1. Emergency Department, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, 445000, China
2. ENT Head and Neck Surgery, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, 445000, China
Abstract
This study assesses whether BMSC from exosome with high miR-184 level ameliorates sepsis. BMSC with high miR-184 expression established. RAW264.7 cells were cultivatedin vitroand divided into control set, model set, BMSC set and BMSC with high miR-184 level set. The model was established through infection of RAW264.7 cells with LPS followed by analysis of cell proliferation and apoptosis, activity of ROS and SOD, secretion of IL-1β, IL-6 and TNF-αas well as the expression of NF-κB and TRAIL. BMSC set showed significantly upregulated miR-184 expression, increased cell proliferation and SOD activity, reduced ROS activity, decreased secretion of IL-1β, IL-6 and TNF-αas well as the expression of NF-κB and TRAIL. The above changes were more significant in the set of BMSC with overexpression of miR-184. In conclusion, cell proliferation, apoptosis and inflammation in RAW264.7 cells induced with LPS is regulated by BMSC from exosome with high expression of miR-184, which is possibly through restraining the NF-κB and TRAIL and oxidative stress.
Publisher
American Scientific Publishers
Subject
Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology
Cited by
1 articles.
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