Affiliation:
1. Department of Nephrology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, Guizhou, China
2. Department of Nephrology, Renhuai Hospital of Traditional Chinese Medicine, Zunyi, 563000, Guizhou, China
Abstract
This study explored specific mechanism of action for polylactic acid-loaded nanoparticles loaded with losartan potassium to regulate the expression of ET-1 in acute kidney injury. Preparation and identification of Polylactic acid nanoparticles (PLA-NP) loaded with losartan potassium
(PLA-NP-LP) were herein performed. After establishment of acute kidney injury rat models, the rats were assigned into model group, PLA-NP group, losartan potassium group, PLA-NP-LP group, NF-kB agonist group, NF-kB inhibitor group, NF-kB agonist and inhibitor group respectively. After one
week of intervention, rat kidney tissues were taken for Hematoxylin and Eosin (H&E) staining and Real-time Polymerase Chain Reaction (PCR) and Western blot were used to measure the levels of NF-kB and ET-1, along with analysis of levels of creatinine and blood urea nitrogen by an automatic
biochemical analyzer. PLA-NP-LP nanoparticles was successfully prepared with good shape. The levels of creatinine and urea nitrogen, renal tubular dilation and epithelial edema in the PLA-NP-LP group were lowest (vs. model group and losartan Potassium group), with P < 0.05. PLA-NP-LP
inhibited the expression of ET-1 and NF-kB, P < 0.05. The ET-1 expression level for rats in the NF-kB agonist combined with PLA-NP-LP group was higher (vs. PLA-NP-LP group). Moreover, the level of ET-1 decreased (P < 0.05). PLA-NP-LP can improve renal function of rats with
acute kidney injury, reduce renal tubular epithelial edema, and downregulate ET-1 in the rat kidney, by inhibiting the expression of NF-kB.
Publisher
American Scientific Publishers
Subject
General Materials Science
Reference26 articles.
1. Acute kidney injury pathology and pathophysiology: A retrospective review;Gaut;Clinical Kidney Journal,2021
2. Acute kidney injury: New concepts in definition, diagnosis, pathophysiology, and treatment;Lattanzio;Journal of the American Osteopathic Association,2009
3. Acute kidney injury: Advances in definition, pathophysiology, and diagnosis;Martin;AACN Advanced Critical Care,2010
4. Sepsis-induced acute kidney injury;Majumdar;Indian Journal of Critical Care Medicine,2010
5. Acute kidney injury: Definition, pathophysiology and clinical phenotypes;Makris;Clinical Biochemist,2016