A Comparative Study of The Effects of Sumac Aqueous and Ethanol Extracts on Candida Albicans in Laboratory Conditions

Abstract

Background and purpose: Candida albicans is an opportunistic pathogenic fungus that is considered a member of the human intestinal flora that can survive outside the human body. This fungus is usually a harmless organism, but it can become pathogenic in immunocompromised people under various conditions. For oral infections, treatment with topical clotrimazole or nystatin is usually effective. In case of failure, oral or intravenous fluconazole, itraconazole or amphotericin B may be used. Several topical antifungal medications may be used for vaginal infections, including clotrimazole. This study was conducted to investigate the effect of sumac aqueous and ethanol extracts on Candida albicans. Methods: In this experimental study, aqueous and alcoholic sumac extracts were prepared and different concentrations were prepared to investigate their effect on Candida albicans. The negative control included the culture medium without mushrooms and the positive control included mushrooms without extract. Candida albicans fungus was cultured after 24, 48 and 72 hours, and control drug concentrations for fluconazole were 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32 on Candida albicans. The effect was given. Methods: In this experimental study, aqueous and alcoholic sumac extracts were prepared and different concentrations were prepared to investigate their effect on Candida albicans. The negative control included the culture medium without mushrooms and the positive control included mushrooms without extract. Candida albicans fungus was cultured after 24, 48 and 72 hours, and control drug concentrations for fluconazole were 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125 millilitres per milligram on Candida albicans. The effect was given. Results: After the cultivation of Candida albicans mushroom and examination, it was found that this mushroom can grow in all concentrations of aqueous extract of the sumac plant and none of the tested concentrations can prevent its growth, and the alcoholic extract at a concentration of 60 mg/ml can inhibit growth. MIC) of Candida albicans fungus, but the fungus was able to grow in other concentrations. The concentration of 0.5 mg/ml of the commercial antibiotic fluconazole could inhibit the growth (MIC) of this fungus. Conclusion: While different concentrations of aqueous and alcoholic extracts of the sumac plant were investigated and it was found that aqueous extract has no effect and alcoholic extract is effective in the same concentration, more and more comprehensive studies are suggested in laboratory scale and living organisms.