Affiliation:
1. Lobachevsky State University of Nizhny Novgorod, Scanning Probe Microscopy Laboratory
2. Nizhny Novgorod State Technical University, Department of Nanotechnology and Biotechnology
Abstract
A two-section analytical system was developed and tested to study the culture of EA.hy926 endothelial cells in real time with high resolution imaging. Scanning ion-conductance microscopy was shown as more relevant method because it didn’t cause mechanical damage of cell, and made possible scanning on the membranes, when endothelial cells were surrounded by nutrient medium. The method allowed not only to analyze changes in the cells morphology, but also to identify extracellular (microfilaments) and intracellular (nucleolus) structures. The rigidity mapping showed that the rigidity of the endotheliocyte membrane varied from 357 to 796 Pa. After 240 min from the beginning of the observation, the formation of endothelial cells apoptotic bodies has begun, and the rigidity of the cell gradually increased, while rigidity of the apoptotic bodies decreased.
Publisher
The Russian Academy of Sciences
Reference15 articles.
1. Горн М.М., Хейтц У.И., Сверинген П.Л., Вебер К.С. 1999. Водно-электролитный и кислотно-основной баланс. СПб.–М.: Невский Диалект, БИНОМ. (Gorn M.M., Heitz W.I., Swearingen P.L., Weber K.S. 1999. Water-electrolyte and acid-base balance. SPb.–M.: Nevsky Dialect, BINOM) (Horne M.M., Heitz U.E., Swearingen P.L. 1991. Fluid, electrolyte, and acid-base balance: a case study approach. St. Louis: Mosby Inc.)
2. Плескова С.Н. 2011. Атомно-силовая микроскопия в биологических и медицинских исследованиях. Долгопрудный: Интеллект. (Pleskova S.N. 2011. Atomic-force microscopy in biology and medicine. Dolgoprudny: Intellect.)
3. Bai M., Grieshaber-Bouyer R., Wang J., Schmider A.B., Wilson Z.S., Zeng L., Halyabar O., Godin M.D., Nguyen H.N., Levescot A., Cunin P., Lefort C.T., Soberman R.J., Nigrovic P.A. 2017. CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood. V. 130. P. 2092.
4. Glasser L., Fiederlein R.L., Huestis D.W. 1985. Liquid preservation of human neutrophils stored in synthetic media at 22 degrees C: controlled observations on storage variables. Blood. V. 66. P. 267.
5. Grzelak K., Łaszcz J., Polkowski J., Mastalski P., Kluczyński J., Łuszczek J., Torzewski J., Szachogłuchowicz I., Szymaniuk R. 2021. Additive manufacturing of plastics used for protection against COVID19 – the influence of chemical disinfection by alcohol on the properties of ABS and PETG polymers. Materials (Basel). V. 14. P. 4823.