Degradation of the Biofilms of Gram-Positive and Gram-Negative Bacteria by the PAPC Serine protease from Aspergillus ochraceus-Reference-Cited by-同舟云学术

Degradation of the Biofilms of Gram-Positive and Gram-Negative Bacteria by the PAPC Serine protease from Aspergillus ochraceus

Published:2024-08-28 Issue:2 Volume:93 Page:228-233
ISSN:0026-3656
Container-title:Микробиология
language:
Short-container-title:Microbiology

Author:

Baidamshina D. R.¹,Rafia Nasr A.¹,Komarevtsev S. K.²,Osmolovskii A. A.³,Miroshnikov K. A.²³,Kayumov A. R.¹,Trizna E. Yu.¹

Affiliation:

1. Kazan (Volga Region) Federal University

2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

3. Moscow State Uniuversity

Abstract

Infections associated with biofilm formation by gram-positive and gram-negative microorganisms cause difficulty in therapy and are prone to transition into chronic forms. Approaches to degradation of the biofilm matrix are therefore in demand. In the present work, recombinant recombinant PAPC serine protease from Aspergillus ochraceus caused the degradation of mature biofilms formed by a number of gram-positive and gram-negative bacteria by 15‒20% at 50 µg/mL. At 100 µg/mL, the biomass of S. aureus and P. aeruginosa biofilms decreased by 50%. Thus, the PAPC may be a promising agent for biofilm removal and enhance the efficiency of antimicrobial therapy.

Publisher

The Russian Academy of Sciences

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