Single-Stage Bioconversion of Phytosterol into Testosterone by Recombinant Strains of Mycolicibacterium neoaurum-Reference-Cited by-同舟云学术

Single-Stage Bioconversion of Phytosterol into Testosterone by Recombinant Strains of Mycolicibacterium neoaurum

Published:2024-08-28 Issue:2 Volume:93 Page:115-121
ISSN:0026-3656
Container-title:Микробиология
language:
Short-container-title:Microbiology

Author:

Tekucheva D. N.¹,Karpov M. V.¹,Fokina V. V.¹,Timakova T. I.¹,Shutov A. A.¹,Donova M. V.¹

Affiliation:

1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences

Abstract

A plasmid containing the genes of a fungal 17β-hydroxysteroid dehydrogenase, which catalyzes the reduction of the steroid core at the C17 position, and mycobacterial glucose-6-phosphate dehydrogenase, which promotes the recycling of the essential coenzyme NAD(P)H, was constructed. Its constitutive expression in well-studied Mycolicibacterium neoaurum strains made it possible to increase significantly the yield of C-17 hydroxysteroids. In particular, recombinant strains created on the basis of M. neoaurum VKM Ac-1815D and M. neoaurum NRRL B-3805 ΔkstD exhibited predominant accumulation of testosterone, while the strain based on M. neoaurum VKM Ac-1816D accumulated dehydrotestosterone and testosterone simultaneously.

Publisher

The Russian Academy of Sciences

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