CBP AND RAD21 BIND AT THE TERMINI OF FORUM DOMAINS IN HUMAN CHROMOSOMES-Reference-Cited by-同舟云学术

CBP AND RAD21 BIND AT THE TERMINI OF FORUM DOMAINS IN HUMAN CHROMOSOMES

Published:2023-11-01 Issue:1 Volume:513 Page:595-598
ISSN:2686-7389
Container-title:Доклады Российской академии наук. Науки о жизни
language:
Short-container-title:Doklady Rossijskoj akademii nauk. Nauki o žizni

Author:

Tchurikov N. A.¹,Alembekov I. R.¹,Klushevskaya E. S.¹,Meilakh P. B.¹,Kretova A. N.¹,Managrova O. D.¹,Kravatskaya G. I.¹,Kravatsky Y. V.¹

Affiliation:

1. Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Abstract

Forum-domains are 50–100-kb stretches of DNA delimited by the hot sport of DSBs. These domains possess coordinately expressed genes. However, molecular mechanisms of such regulation are not clear. It is supposed that the proteins specifically binding at the termini of domains could be involved in coordinated regulation of expression. In this study we used the results of precise mapping of hot spots of DSBs and ChIP-Seq data for ten nuclear proteins in HEK293T cell line for a search of proteins specifically binding at forum-domains termini. We detected that two proteins – CBP and RAD24-, which are known to be involved in epigenetic regulation of gene expression and formation of 3D chromosomal structures, bind at the termini. We suppose that these proteins could be involved in coordinated expression of genes in forum-domains.

Publisher

The Russian Academy of Sciences

Reference15 articles.

1. Tchurikov N.A., Kretova O.V., Sosin D.V., Zykov I.A., Zhimulev I.F., Kravatsky Y.V. // Genome-wide profiling of forum domains in Drosophila melanogaster. Nucleic Acids Res. 2011, 39, 3667–3685.

2. Tchurikov N., Krasnov A.N., Ponomarenko N.A., Golova Y.B., Chernov B.K. // Forum domain in Drosophila melanogaster cut locus possesses looped domains inside. Nucleic Acids Res. 1998, 26, 3221–3227.

3. Tchurikov N.A., Kretova O.V., Fedoseeva D.M., Sosin D.V., Grachev S.A., Serebraykova M.V., Romanenko S.A., Vorobieva N.V., Kravatsky Y.V. // DNA Double-Strand Breaks Coupled with PARP1 and HNRNPA2B1 Binding Sites Flank Coordinately Expressed Domains in Human Chromosomes. PloS Genet. 2013. V. 9. P. e1003429.

4. Dudás A., Chovanec M. // DNA double-strand break repair by homologous recombination. Mutat Res. 2004. V. 566 (2), 131–167.

5. Vilenchik M.M., Knudson A.G. // Radiation dose-rate effects, endogenous DNA damage, and signaling resonance. Proc. Natl.Acad. Sci. USA 2006, V. 103. P. 17874–17879.