Participation of the transcription factor CREB1 in the regulation of the Mdh2 gene encoding malate dehydrogenase in the liver of rats with alloxan diabetes

Abstract

The aim of the study was to study the role of transcription factor CREB1 in regulating the expression of the gene encoding the mitochondrial form of malate dehydrogenase (MDH, EC 1.1.1.37) in the liver of rats with experimental diabetes. An increase in the rate of work of NAD-dependent malate dehydrogenase in rat liver cells during the development of experimental diabetes was shown, associated with the activation of the Mdh1 and Mdh2 genes encoding this enzyme. The analysis of the promoters of these genes showed that only in the Mdh2 gene there is a specific binding site with the transcription factor CREB1. It was found that in the liver of rats with pathology, there is an increase in the rate of expression of the gene encoding this transcription factor, which correlates with the expression of the Mdh2 gene. Thus, the data obtained by us confirm the possibility of positive regulation of the rate of the Mdh2 gene by the transcription factor CREB1.