Kinetics and mechanism of inhibition of horseradish peroxidase activity by N-phosphonomethyl-glycine-Reference-Cited by-同舟云学术

Kinetics and mechanism of inhibition of horseradish peroxidase activity by N-phosphonomethyl-glycine

Published:2023-06-15 Issue:3 Volume:68 Page:442-450
ISSN:0006-3029
Container-title:Биофизика
language:
Short-container-title:Biofizika

Author:

Avdeeva L. V¹,Vakhterova Ya. V²,Saratovskikh E. A¹

Affiliation:

1. Federal Research Center for Problems of Chemical Physics and Medical Chemistry, Russian Academy of Sciences

2. Lomonosov Moscow State University

Abstract

The inhibition of horseradish peroxidase activity by N-phosphonomethyl-glycine, an active ingredient in the most extensively used glyphosate-based herbicide (Roundup) in agricultural applications worldwide, was investigated. It has been shown that N-phosphonomethyl-glycine inhibits the activity of peroxidase from horseradish roots. This inhibition is of a competitive nature, the value of Ki = 0.1004 mM. The Michaelis constant (Km = 0.1776 mM) and herbicide concentration (I50 of Roundup = 0.302 mM) were determined. The results demonstrate that N-phosphonomethyl-glycine can suppress the activity of the antioxidant system, and in warm-blooded organisms it may cause the accumulation of free radicals, thereby contributing to expanding knowledge of biological targets Roundup is applied to.

Publisher

The Russian Academy of Sciences

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