Computer Simulation of Short DNA Fragments Induced by HIGH-LET Charged Particles-Reference-Cited by-同舟云学术

Computer Simulation of Short DNA Fragments Induced by HIGH-LET Charged Particles

Published:2024-12-15 Issue:2 Volume:69 Page:
ISSN:0006-3029
Container-title:Биофизика
language:
Short-container-title:Biofizika

Author:

Eidelman Y. A¹²,Salnikov I. V¹,Andreev S. G¹²

Affiliation:

1. N.M. Emanuel Institute of Biochemical Physics

2. National Research Nuclear University MEPhI

Abstract

The formation of short DNA fragments, up to 3 kbp, induced in chromatin by nitrogen (LET 97 keV/mм) and iron (LET 190 keV/mм) ions was studied by computer simulation. Chromatin models with different structure parameters and Monte Carlo track structure simulation were used to assess the impact of chromatin fiber structure and LET on the DNA fragment size distribution. For the structures modeled (different types of solenoids, a chain of nucleosomes), the fragment size distribution had a maximum in the region of ~100 bp corresponding to the formation of DNA breaks in two neighboring turns of the helix on the nucleosome. The calculation predicted the peak in the region of ~1000 bp, corresponding to the formation of DNA breaks in two neighboring turns of the solenoid, which parameters depended on the degree of compactness of the fiber and were independent of LET. The assumption was introduced of the presence of subpopulations of various chromatin structures under irradiation. It allowed to explain the experimentally observed size distributions of the short DNA fragments induced by high-LET charged particles.

Publisher

The Russian Academy of Sciences

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