Interferon α2b As a Modulator of the Afferent Glutamatergic Synapse of the Frog Vestibular Apparatus

I. V. Ryzhova; Рыжова И. В.; E. A. Korneva; Корнева Е. А.; T. V. Tobias; Тобиас Т. В.; E. A. Protasov; Протасов Е. А.; E. A. Vershinina; Вершинина Е. А.

doi:10.31857/S0233475523060087

Interferon α2b As a Modulator of the Afferent Glutamatergic Synapse of the Frog Vestibular Apparatus

Authors: Ryzhova I.V.¹, Korneva E.A.², Tobias T.V.¹, Protasov E.A.², Vershinina E.A.¹
Affiliations:
1. Pavlov Institute of Physiology, Russian Academy of Sciences
2. Institute of Experimental Medicine
Issue: Vol 40, No 6 (2023)
Pages: 463-471
Section: Articles
URL: https://journals.rcsi.science/0233-4755/article/view/148095
DOI: https://doi.org/10.31857/S0233475523060087
EDN: https://elibrary.ru/GLDJXD
ID: 148095

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Abstract
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Abstract

Reportedly, the innate and adaptive immunity molecules can modulate the synaptic activity of the central nervous system. Interferons are widely used in the treatment of oncological and viral diseases. Even though interferons are classified as ototoxic substances, the mechanism of their effect on the synaptic activity of the inner ear remains unexplored. Here, we analyzed modulating influences of interferon α2b (IFN-α2b) on the function of afferent glutamatergic synapse in the conditions of drug application to the synaptic zone. The experiments were performed on the isolated vestibular apparatus of a frog (Rana temporaria) using an electrophysiological method. IFN-α2b (0.2–40 ng/mL) caused an increase in the background pulse activity of afferent fibers, followed by a decrease in the frequency of discharges, usually at high concentrations of the interferon. Besides, IFN-α2b decreased the glutamate (L-Glu) evoked response and modulated the level of afferent fiber activity restored by L-Glu under the conditions of blockade of the glutamate release from hair cells in hyper-Mg²⁺ and hypo-Ca²⁺ solution. This suggests the postsynaptic effect of IFN-α2b. The presented data indicate the neuromodulating effect of interferon on the synaptic activity of the afferent synapse of the vestibular apparatus.

Keywords

pro-inflammatory cytokines, interferon, vestibular apparatus, hair cell, glutamatergic synapse, neuroimmunomodulation

References

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