Effects of Human Chorionic Gonadotropin on Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells

K. Yu. Shardina; Шардина К. Ю.; V. P. Timganova; Тимганова В. П.; M. S. Bochkova; Бочкова М. С.; S. V. Uzhviyuk; Ужвиюк С. В.; S. A. Zamorina; Заморина С. А.

doi:10.31857/S0233475523050092

Effects of Human Chorionic Gonadotropin on Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells

Authors: Shardina K.Y.¹, Timganova V.P.¹, Bochkova M.S.¹, Uzhviyuk S.V.¹, Zamorina S.A.¹
Affiliations:
1. Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences
Issue: Vol 40, No 5 (2023)
Pages: 404-412
Section: Articles
URL: https://journals.rcsi.science/0233-4755/article/view/135047
DOI: https://doi.org/10.31857/S0233475523050092
EDN: https://elibrary.ru/THVNKD
ID: 135047

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Abstract
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Abstract

The effect of recombinant human chorionic gonadotropin (hCG) at concentrations of 10 and 100 MU/mL, typical for pregnancy, on differentiation and functional activity of myeloid-derived suppressor cells (MDSCs) was investigated. The subject of the study was isolated cells CD11b⁺ that acquired the MDSC phenotype as a result of two-step activation with cytokines GM-CSF and IL-1β and lipopolysaccharide (LPS). It was shown that hCG at both concentrations significantly increased the total MDSC pool and at a lower concentration (10 IU/mL) promoted differentiation of the M-MDSC subpopulation. At the same time, 100 MU/mL hCG had no effect on the expression of arginase-1 and indolamine-2,3-dioxygenase (IDO) in MDSCs, but at a concentration of 10 IU/mL there was a tendency to increase IDO expression under the influence of hCG. When the cytokine profile was evaluated by multiplex analysis using Luminex xMAP technology, it was found that hCG did not modulate cytokine production in the CD11b⁺ cell culture. Thus, this work demonstrates for the first time that hCG can induce MDSC differentiation.

Keywords

myeloid-derived suppressor cells (MDSCs), in vitro cultivation, human chorionic gonadotropin (hCG), CD11b⁺ cells, IDO, Arg1

References

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