Validation of a rapid test for HLA-B*58:01/57:01 allele screening to detect individuals at risk for drug-induced hypersensitivity-Reference-Cited by-同舟云学术

Validation of a rapid test for HLA-B*58:01/57:01 allele screening to detect individuals at risk for drug-induced hypersensitivity

Published:2016-04 Issue:5 Volume:17 Page:473-480
ISSN:1462-2416
Container-title:Pharmacogenomics
language:en
Short-container-title:Pharmacogenomics

Author:

Nguyen Dinh Van¹²³,Vidal Christopher¹,Li Jamma¹,Fulton Richard B¹,Fernando Suran L¹²

Affiliation:

1. ImmunoRheumatology Laboratory, Pathology North – Northern Sydney, St Leonards, Australia

2. Sydney Medical School – Northern, University of Sydney, Sydney, Australia

3. Department of Allergy & Clinical Immunology, Hanoi Medical University, Hanoi, Vietnam

Abstract

Aim: In prevention of allopurinol and abacavir hypersensitivity, screening HLA-B*58:01/57:01 has been highly recommended prior to commencing these therapies. Therefore, we aimed at developing and validating a rapid and robust screening method for HLA-B*58:01/57:01. Materials & methods: Real-time polymerase chain reaction with TaqMan® probes was employed to detect HLA-B*58:01/57:01. Results: The newly developed assay has the sensitivity of 100% (95% CI: 79.4–100.0%), the specificity of 98.8% (95% CI: 93.6–99.9%), the positive predictive value of 94.1% (95% CI: 71.3–99.9%) and the negative predictive value of 100.0% (95% CI: 95.7–100.0%). The lowest limit of detection is 0.04 ng/µl of DNA. Conclusion: The present method is a rapid and robust assay that is appropriate for screening of HLA-B*58:01/*57:01 alleles.

Publisher

Future Medicine Ltd

Subject

Pharmacology,Genetics,Molecular Medicine

Link

https://www.futuremedicine.com/doi/pdf/10.2217/pgs.15.185

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