Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible <i>Staphylococcus aureus</i>-Reference-Cited by-同舟云学术

Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible Staphylococcus aureus

Published:2024-01-31 Issue: Volume: Page:
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Stefany Aires do Nascimento Francisca B¹²^ORCID,do Amaral Valente Sá Lívia Gurgel¹²³^ORCID,de Andrade Neto João B¹²³^ORCID,da Silva Lisandra Juvêncio¹²^ORCID,Rodrigues Daniel Sampaio¹²^ORCID,de Farias Cabral Vitória P¹²^ORCID,Barbosa Amanda Dias¹²^ORCID,Almeida Moreira Lara E¹²^ORCID,Braga Vasconcelos Camille R¹²,Cavalcanti Bruno Coêlho²⁴^ORCID,França Rios Maria E²⁴,Silva Jacilene⁵^ORCID,Marinho Emmanuel Silva⁵^ORCID,dos Santos Helcio Silva⁶^ORCID,de Mesquita Jacó RL⁷,Pinto Lobo Marina Duarte⁸^ORCID,de Moraes Manoel Odorico²⁴^ORCID,Nobre Júnior Hélio V¹²^ORCID,da Silva Cecília Rocha¹²^ORCID

Affiliation:

1. School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, 60430-372, Brazil

2. Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil

3. Christus University Center (UNICHRISTUS), Fortaleza, CE, 60190-180, Brazil

4. Department of Physiology & Pharmacology, Federal University of Ceará, Fortaleza, CE, 60430-275, Brazil

5. Department of Chemistry, Group of Theoretical Chemistry & Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, 62930-000, Brazil

6. Science & Technology Centre, Course of Chemistry, State University Vale do Acaraú, Sobral, CE, 62010-560, Brazil

7. St. Joseph Hospital for Infectious Diseases, Fortaleza, CE, 60455-610, Brazil

8. Department of Biology, Federal University of Ceará, Fortaleza, CE, 60440-900, Brazil

Abstract

Background: Staphylococcus aureus is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. Materials & methods: The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. Results: MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. Conclusion: Hydralazine is a potential antibacterial.

Publisher

Informa UK Limited

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2023-0160

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