Evaluation of the genotoxicity of diflubenzuron by micronucleus test on red blood cells Danio rerio
Author:
Medyankina Mariya1, Kochetkov Nikita2, Golovacheva Natalya2, Nikiforov-Nikishin Dmitry32
Affiliation:
1. MGUTU named after K.G. Razumovsky 2. Moscow State University of Technologies and Management (FCU) 3. Moscow state University University of technology and managing it. Kg Razumovsky (PKU)
Abstract
In this paper, the genotoxicity of (1-(4-chlorophenyl)-3-(2,6-difluoro-benzoyl)urea) is investigated by a micronuclear test on Danio rerio, as a standard test object, at concentrations of 0.5, 1 and 2 mg/l. As a result of the work, a significant increase in the frequency of occurrence of micronuclei (0.73%) was found, while other nuclear anomalies in the maximum concentrations of erythrocytes were also significant. It was found that the frequency of micronuclei in concentrations of 0.5 and 1 mg/l on the fifth day of the experiment was the maximum, while at the maximum concentration (2 mg/l) the level of micronuclei was lower, which is probably due to toxic effects. An increase in the level of micronuclei may be associated with the genotoxic effect of DFB decay products. The genotoxicity results obtained using the micronucleus test method were contradictory. For this reason, it is necessary to conduct additional studies using the comet method or experiments on cell cultures.
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