Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)-Reference-Cited by-同舟云学术

Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)

Published:2004-09 Issue:5 Volume:47 Page:685-691
ISSN:1516-8913
Container-title:Brazilian Archives of Biology and Technology
language:
Short-container-title:Braz. arch. biol. technol.

Author:

Sarubbo Leonie Asfora¹,Oliveira Luciana Alves de²,Porto Ana Lúcia Figueiredo³,Campos-Takaki Galba Maria de¹,Tambourgi Elias Basile²

Affiliation:

1. Universidade Católica de Pernambuco, Brazil

2. UNICAMP, Brazil

3. UFPE, Brazil; UFRPE, Brazil

Abstract

The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.

Publisher

FapUNIFESP (SciELO)

Subject

Multidisciplinary

Link

http://www.scielo.br/pdf/babt/v47n5/a03v47n5.pdf

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