Epidemiological survey on Mycoplasma gallisepticum and M. synoviae by multiplex PCR in commercial poultry

Author:

Buim Marcos Roberto1,Mettifogo Elena2,Timenetsky Jorge2,Kleven Stanley3,Ferreira Antonio J. Piantino4

Affiliation:

1. Instituto Biológico de São Paulo, Brazil

2. Universidade de São Paulo, Brazil

3. University of Georgia, USA

4. USP, Brazil

Abstract

Mycoplasmas are important avian pathogens, which cause respiratory and joint diseases that result in large economic losses in Brazilian and world-wide poultry industry. This investigation regarding the main species of mycoplasmas, Mycoplasma gallisepticum (MG) and M. synoviae (MS), responsible for the above mentioned conditions, was carried out through PCR Multiplex analysis. One thousand and forty-six (1,046) samples of tracheal swabs and piped embryos were collected from 33 farms with laying hens, breeders, broilers or hatchery, located in the Brazilian states of São Paulo, Paraná and Pernambuco, where respiratory problems or drops in egg production had occurred. The MG and MS prevalence on the farms was 72.7%. These results indicated (1) high dissemination of mycoplasmas in the evaluated farms, with predominance of MS, either as single infectious agent or associated with other mycoplasmas in 20 farms (60.6%), and (2) an increase of MS and decrease of MG infection in Brazilian commercial poultry.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

Reference22 articles.

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2. Application of polymerase chain reaction with arbitrary primers to strain identification of Mycoplasma gallisepticum;Fan H.H.;Avian Dis.,1995

3. A medium for the isolation of avian mycoplasmas;Frey M.L.;Am. J. Vet. Res.,1968

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