Detection of blaCTX-M genes in ESBL-producing Klebsiella isolates from animals in Croatia-Reference-Cited by-同舟云学术

Detection of blaCTX-M genes in ESBL-producing Klebsiella isolates from animals in Croatia

Published:2023-06-20 Issue:1 Volume:71 Page:12-15
ISSN:0236-6290
Container-title:Acta Veterinaria Hungarica
language:
Short-container-title:AVet

Author:

Pintarić Selma¹,Štritof Zrinka¹^ORCID,Perko Vesna Mojčec¹,Tumpa Andrea²,Cvetnić Marija¹,Hadžić Lana³

Affiliation:

1. Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia

2. Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia

3. Laboratory for Food, Water and Animal Feed Microbiology, PI ‘Veterinary Institute’, Bihać, Bosnia and Herzegovina

Abstract

AbstractThis study investigated the frequency of third-generation cephalosporin resistance in Klebsiella spp. isolated from animals in Croatia and the presence of blaCTX-M genes. A total of 711 enteric bacteria were isolated from clinical samples, and Klebsiella spp. isolates accounted for 6.9% (n = 49). Thirteen Klebsiella isolates (26.5%) were ESBL producers, nine isolates from the Klebsiella pneumoniae species complex (69.2%), and four (30.8%) Klebsiella oxytoca isolates. All carried the blaCTX-M-15 gene, and antimicrobial susceptibility testing revealed them as multidrug resistant. All were resistant to all tested cephalosporins, fluoroquinolones, aminoglycosides and aztreonam, 92.3% showed resistance to tetracycline, 84.6% to trimethoprim-sulfamethoxazole and 69.2% to nitrofurantoin. No isolate showed resistance to imipenem and meropenem. It can be concluded that ESBL-producing Klebsiella expressing the blaCTX-M gene are not rare among Klebsiella isolates from animals in Croatia.

Publisher

Akademiai Kiado Zrt.

Subject

General Veterinary

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