THE EFFECTS OF EXERCISE FATIGUE ON MGLUR1 EXPRESSION AND OXIDATIVE STRESS IN STRIATUM OF RAT

Meihua Su¹, Dan Xu², Jianmin Chen^{1, *}

¹School of Physical Education, Jimei University, Xiamen- 361021, China - ²College of P. E. and Sports, Beijing Normal University, Beijing - 100875, China

Objective: It aims to observe the changes of mGluR1 in striatum of rats induced by repeated exhaustive exercise and to discuss the effects of mGluR1 and oxidative damage played in the exercise fatigue. 

Methods: There were 48 male wistar rats, which were randomly divided into four groups including CG, 0EG, 24EG and 48 EG, each group having 12 rats. Meanwhile, immunohistochemistry (IMM) technique was used to investigate the expression of positive cell and integrated optical density (IOD) of metabotropic glutamate receptor (mGluR1), and the impact of exhaustive exercise on malondialdehyde (MDA),Glutathione peroxidase (GSH-PX or GPX) and superoxide dismutase (SOD) expression in rats brain were also investigated in different groups. 

Results: Compared with CG, the expression of mGluR1 protein of striatum in 0EG and 24EG both significantly increased, and the value of mGluR1 reduced to the level of control group. Meanwhile, the SOD activities of 0EG and 24EG group were significantly higher than the control group, and it was the same with GSH-PX in brain tissue of rats. Meanwhile, MDA has been investigated that it increased significantly in 0EG and 24EG compared with control group, and the MDA level of 48EG was still significant higher than CG. 

Conclusion: R: Repeated exhaustive exercise could result in up-regulation of mGluR1 and rising activity of SOD, GPX and MDA in striatum of rats, which suggested that mGluR1 was another important receptor related with the increase of free radicals induced by exercise fatigue.