Author:
Bezerra-Santos Márcio,Bomfim Lays G. Santos,Santos Camilla N. Oliveira,Cunha Maria Wiliane N.,de Moraes Eduardo J. Rocha,Cazzaniga Rodrigo A.,Tenório Martha D. L.,Araujo Jonnia M. Sherlock,Menezes-Silva Lucas,Magalhães Lucas Sousa,Barreto Aline S.,Reed Steven G.,Duthie Malcolm S.,Lipscomb Michael W.,de Almeida Roque Pacheco,de Moura Tatiana Rodrigues,de Jesus Amélia Ribeiro
Abstract
Leprosy reaction (LR) and physical disability (PD) are the most significant clinical complications of leprosy. Herein, we assessed the circulating serum-sTREM-1 and TNF-α levels and their genetic polymorphisms in leprosy. Serum-sTREM-1 and TNF-α levels were measured in leprosy patients (LP) before treatment (n = 51) and from their household contacts (HHCs; n = 25). DNA samples were genotyped using TREM-1 rs2234246 and TNF-α rs1800629-SNP in 210 LPs and 168 endemic controls. The circulating sTREM-1 and TNF-α levels are higher in the multibacillary form. The ROC curve of the serum-sTREM-1 levels was able to differentiate LR from non-LR and PD from non-PD. Similarly, LPs with serum-sTREM-1 levels >210 pg/ml have 3-fold and 6-fold higher chances of presenting with LR and PD, respectively. Genotypes CC+CT of the TREM-1 were associated with leprosy. Taken together, our analyses indicated that sTREM-1 and TNF-α play an important role in the pathogenesis of leprosy and provide promising biomarkers to assist in the diagnosis of leprosy complications.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Apoio à Pesquisa e à Inovação Tecnológica do Estado de Sergipe