Author:
Xufré Cristina,González Tanía,Leal Lorna,Trubey Charles M.,Lifson Jeffrey D.,Gatell José María,Alcamí José,Climent Núria,García Felipe,Sánchez-Palomino Sonsoles
Abstract
We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.
Funder
Ministerio de Economía y Competitividad
European Regional Development Fund
Instituto de Salud Carlos III
Generalitat de Catalunya
National Cancer Institute
“la Caixa” Foundation