Author:
Wang Ziyi,Wang Xuesong,Guo Zhe,Liao Haiyan,Chai Yan,Wang Ziwen,Wang Zhong
Abstract
The molecular mechanism of Reduning (RDN) in the treatment of sepsis was analyzed based on network pharmacology. The system pharmacology method was administered to search the active ingredients and targets of RDN, identify the sepsis-related genes, and determine the targets of RDN in the treatment of sepsis. Cytoscape was used to build a “drug component-target” network to screen key compounds. A protein-protein interaction (PPI) network was constructed using STRING, and core targets were revealed through topological analysis. 404 shared targets of RDN and sepsis were introduced into DAVID Bioinformatics Resources 6.8 for GO and KEGG enrichment analysis to predict their possible signaling pathways and explore their molecular mechanisms. GO enrichment analysis highlighted that they were largely related to protein phosphorylation, inflammatory reaction, and positive regulation of mitogen-activated protein kinase (MAPK) cascade. KEGG enrichment analysis outlined that they were enriched in PI3K-AKT signaling pathway, calcium signaling pathway, rhoptry-associated protein 1 (Rap1) signaling pathway, and advanced glycation end products and receptors for advanced glycation end products (AGE-RAGE) signaling pathway. Molecular biological validation results exposed that RDN could significantly improve the protein expression of p-AKT and p-PI3K, alleviate apoptosis-related proteins expression level and decrease apoptosis rate in LPS-induced HUVECs. In conclusion, it was illustrated that RDN could considerably constrain LPS-induced apoptosis by activating the PI3K-AKT signaling pathway, which advocated a basis for fundamental mechanism research and clinical application of RDN in the treatment of sepsis.
Subject
Pharmacology (medical),Pharmacology
Reference38 articles.
1. ICU-mortality in Old and Very Old Patients Suffering from Sepsis and Septic Shock;Bruno;Front. Med. (Lausanne),2021
2. Effect of Reduening Injection Adjuvant Therapy on Inflammatory Indexes and Immune Function in Patients with Acute Cholecystitis [J];Chen;J. Clin. Emerg.,2021
3. Epidemiology of Sepsis and Septic Shock;Chiu;Curr. Opin. Anaesthesiol.,2021
4. Gardenia Decoction Prevent Intestinal Mucosal Injury by Inhibiting Pro-inflammatory Cytokines and NF-Κb Signaling;Cui;Front. Pharmacol.,2019
5. Effect of Reduining Combined with Five-Dimensional Lysine Granules on Serum cTnI and CK-MB Levels in the Treatment of Hand-Foot-Mouth Disease [J];Dai;Chin. J. Traditional Chin. Med.,2021
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