Author:
Lin Jiasheng,Li Sanhua,Ye Benchen,Zheng Weigang,Wang Huihui,Liu Ying,Wang Dong,Wu Zaihui,Dong Wen-Fei,Zan Minghui
Abstract
Introduction: Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance.Methods: To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip.Results: The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30–2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%.Discussion: The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.
Funder
China Postdoctoral Science Foundation
National Key Research and Development Program of China
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology