Author:
Hensen Luca,Illing Patricia T.,Rowntree Louise C.,Davies Jane,Miller Adrian,Tong Steven Y. C.,Habel Jennifer R.,van de Sandt Carolien E.,Flanagan Katie L.,Purcell Anthony W.,Kedzierska Katherine,Clemens E. Bridie
Abstract
CD8+T cells are a pivotal part of the immune response to viruses, playing a key role in disease outcome and providing long-lasting immunity to conserved pathogen epitopes. Understanding CD8+T cell immunity in humans is complex due to CD8+T cell restriction by highly polymorphic Human Leukocyte Antigen (HLA) proteins, requiring T cell epitopes to be defined for different HLA allotypes across different ethnicities. Here we evaluate strategies that have been developed to facilitate epitope identification and study immunogenic T cell responses. We describe an immunopeptidomics approach to sequence HLA-bound peptides presented on virus-infected cells by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Using antigen presenting cell lines that stably express the HLA alleles characteristic of Indigenous Australians, this approach has been successfully used to comprehensively identify influenza-specific CD8+T cell epitopes restricted by HLA allotypes predominant in Indigenous Australians, including HLA-A*24:02 and HLA-A*11:01. This is an essential step in ensuring high vaccine coverage and efficacy in Indigenous populations globally, known to be at high risk from influenza disease and other respiratory infections.
Funder
National Health and Medical Research Council
Subject
Immunology,Immunology and Allergy
Cited by
4 articles.
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