Author:
Nirmala G. N.,Sharma Akshata,Ragunathan Venkataraghavan
Abstract
This study examined the aquatic toxicity of dichloromethane (DCM) on Oreochromis mossambicus. Along with aquatic toxicity studies, in silico research was also conducted to identify ways of improving the fish’s immune system, which may help to fight the oxidative stress and neurotoxic effects of DCM. The activities of glutathione S-transferase and acetylcholinesterase enzymes were studied in samples from the brains, muscles, livers, and gills of fish treated with different concentrations of DCM (730, 760, and 790 ppm). Histopathological and hematological studies were also completed at various concentrations of DCM. Molecular docking studies of the bioactive compounds of Aloe vera against interleukin-1β (IL-1β) were conducted, and drug properties were also analyzed. The lethal concentration (LC50) of DCM in the fish was found to be 760 ppm. The hematological study revealed that tissues exposed to 760 ppm of DCM had an elevated leukocyte count, high amounts of hemoglobin, and very low platelet counts. The liver histopathological study identified cellular alterations such as necrosis, and the gills showed lamellar fusion and congestion. The compound sitosterol showed strong binding energy (-12.398 kcal/mol) against IL-1β, followed by squalene (−12.157 kcal/mol). Pharmacokinetic properties were also analyzed, with satisfactory results. Thus, the hematological and histopathological studies reveal that DCM has a potential ability to induce oxidative stress and neurotoxic effects. Hence, the phytochemicals of Aloe vera can improve the immune system to fight against the neurotoxicity of DCM, which can be further validated by in vitro and in vivo studies.
Subject
General Environmental Science
Reference54 articles.
1. A Structure Based Molecular Docking of Acacia Catechu against Contractile Protein Plasmodium Falciparum – a Malarial Disease;Abhishek Biswal;Int. J. Pharm. Sci. Drug Res. November-December,2020
2. Molecular docking and ADMET studies of bioactive compounds of rhizopora mucornata AGAINST bacterial enzyme protein tyrosine phosphatase;Abhishek Biswal;Int. J. Pharm. Sci. Res.,2020
3. Haematological Studies of Freshwater Fish, Labeo Rohita (ham.) Exposed to Heavy Metals;Ahmad;Int. J. Sci. Environ. Technol.,2015
4. Analysis of phytochemical constituents and antimicrobial activities of Aloe Vera L. Against clinical pathogens;Arunkumar;World J. Agric. Sci.,2009
5. Biochemical and Subcellular Changes in Carp Exposed to the Organophosphorus Methidathion and the Pyrethroid Deltamethrin;Balint;Aquat. Toxicol.,1995
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