Effects of Lactiplantibacillus plantarum 19-2 on immunomodulatory function and gut microbiota in mice

Abstract

This study aims to evaluate the effects ofLactiplantibacillus plantarum19-2 (L. plantarum19-2) on mice treated with the alkylating agent cyclophosphamide (CTX). Our findings show thatL. plantarum19-2 restored the spleen and thymus index and the number of white blood cells and lymphocytes% in CTX treated mice. Serum immunoglobulin levels in CTX-treated mice were increased byL. plantarum19-2. In addition, as compared to the model group,L. plantarum19-2 upregulated the content of SIgA, whileL. plantarum19-2 regulates the mRNA and protein expression levels of GATA-3, T-bet, IFN-γ, and IL-4 in small intestinal tissues, which adjusted mucosal barriers, structural status, and the balance of Helper T-cell 1 and Helper T-cell 2.Lactiplantibacillus plantarum19-2 regulated the distribution of intestinal flora in mice, promoting the growth ofBacteroidesandProteobacteria. In addition,L. plantarum19-2 inhibited the growth of several harmful bacteria, includingActinobacteriaandFirmicutes.