Author:
Xing Jun-Hong,Shi Chun-Wei,Sun Ming-Jie,Gu Wei,Zhang Rong-Rong,Chen Hong-Liang,Li Ying,Wang Dan,Li JunYi,Niu Tian-Ming,Huang Qun-Tao,Qian Jia-Hao,Huang Hai Bin,Jiang Yan-Long,Wang Jian-Zhong,Cao Xin,Wang Nan,Zeng Yan,Yang Gui-Lian,Yang Wen Tao,Wang Chun-Feng
Abstract
There are some limitations of traditional influenza vaccines concerning novel mutant strains. Therefore, it is particularly important to develop preventive means for antigen-unrelated types of influenza viruses. Recent studies have shown that probiotics can modulate the immune system and reduce the severity of viral infections. In this study, we investigated the potential ofLactiplantibacillus plantarum0111 against influenza virus H9N2. Challenge experiments showed thatL. plantarum0111 pretreatments could effectively improve mice’s survival rate and weight loss and reduce the inflammatory cytokines IL-6 and TNF-α in the lungs and bronchoalveolar lavage fluid (BALF) along with the degree of lung and intestinal injury. FMT experiment demonstrates that the protective effect produced byL. plantarum0111 is associated with gut microorganisms. In addition, 16S high-throughput sequencing of the mouse intestinal microbiota showed thatL. plantarum0111 remodeled the intestinal microbiota after H9N2 infection and maintained the gut microbiota balance. In a mouse model, the oral administration ofL. plantarum0111 increased IFN-β expression in the serum and BALF. At the same time, the transcript levels of IFN-β and related ISGs in the intestine and lungs of mice were also increased. In addition, the activation and polarization of T cells in mesenteric lymph nodes (MLNs) and the spleen were detected by flow cytometry, and the results showed thatL. plantarum0111 modulated cytokines in T cells and increased IgA expression in B cells in the MLNs and spleen. Thus,L. plantarum0111 may improve gut microbiota-mediated immune responses and thus, resist infection by the influenza virus, and it could be used as an effective preventive measure against the influenza virus.
Funder
National Natural Science Foundation of China
Research and Development
Subject
Microbiology (medical),Microbiology
Cited by
4 articles.
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