The Role of BMP7 in the Proliferation of Hu Sheep Dermal Papilla Cells Is Influenced by DNA Methylation

Author:

Lv Xiaoyang123,He Mingliang2ORCID,Wang Shanhe23ORCID,Zheng Wenxin4,Zhou Hanlin5,Mwacharo Joram M.6,Sun Wei123ORCID

Affiliation:

1. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China

2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

3. International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou University, Yangzhou 225009, China

4. Xinjiang Academy of Animal Sciences, Urumqi 830011, China

5. Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524000, China

6. International Centre for Agricultural Research in the Dry Areas, Addis Ababa 999047, Ethiopia

Abstract

Previous studies have shown that the BMP7 gene is differentially expressed in Hu sheep lamb skin of different pattern types, and its expression level is significantly correlated with hair follicle indices of different pattern types, but the molecular mechanism of the differential expression of the BMP7 gene remains unclear. This study investigated the effect of DNA methylation on the transcriptional expression of BMP7. Firstly, we found that the mRNA expression of the BMP7 gene and the activity of the core promoter of the BMP7 gene were upregulated after 5-Aza-Deoxycytidine-induced demethylation treatment using qRT-PCR and double luciferase reporter assay. Then, we found that the proliferation of Hu sheep DPCs in vitro was promoted after 5-Aza-Deoxycytidine-induced demethylation treatment through qRT-PCR, CCK-8, and EdU assay, and that the overexpression of DNMT1 in DPCs induced the opposite effect. In addition, the results of the cell cycle assay reveal that the percentage of cells in the S phase was increased after 5-Aza-Deoxycytidine-induced demethylation treatment, and that the percentage of cells in the S phase was decreased after overexpression of DNMT1 in DPCs. This study indicated that the differential expression of the BMP7 gene in different patterns of Hu sheep lamb skin may be regulated by DNA methylation modification. In addition, DNA methylation can regulate the proliferation and cell cycle of DPCs in Hu sheep.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Major Project of the Natural Science Foundation of Xinjiang Uyghur Autonomous Region

Project of Jiangsu Innovation Support Program for International Science and Technology Cooperation—“Belt and Road” Innovation Cooperation

Jiangsu Agricultural Science and Technology Innovation Fund

Natural Science Foundation of the Jiangsu Higher Education Institutions of China

Jiangsu 333 Distinguished Talents Project Foundation

Science and Technology Plan Project of Yangzhou

Publisher

MDPI AG

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