In Vitro and Ex Vivo Models for Screening Topical Anti-Inflammatory Drugs

Author:

Pérez-Salas Juan Luis1,Moreno-Jiménez Martha Rocío1ORCID,Rocha-Guzmán Nuria Elizabeth1,González-Laredo Rubén Francisco1,Medina-Torres Luis2ORCID,Gallegos-Infante José Alberto1ORCID

Affiliation:

1. TecNM/Instituto Tecnológico de Durango, UPIDET, Blvd Felipe Pescador 1830 Ote, Col Nueva Vizcaya, Durango 34080, Mexico

2. Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico

Abstract

Skin inflammation occurs as an immune response to various stimuli such as ultraviolet light, irritants, or any type of skin barrier injury. Finding safe and effective drugs to combat skin inflammation remains a research challenge. Ethical and legal considerations in animal testing encourage the development of in vitro and ex vivo models for the detection of skin inflammation. This report presents an updated review of non-animal study models available for screening drugs with anti-inflammatory potential. It includes a description of the basic methods used to inhibit protein denaturation and red blood cell membrane stability. Three in vitro inhibition assay methods for enzymes relevant to the skin inflammatory process are then described. The development of cell culture models is described: relatively simple and easy-to-produce two-dimensional (2D) skin cell cultures that allow assessment of response to a given stimulus, three-dimensional (3D) cell cultures that better mimic human skin physiology by more accurately replicating mechanical and chemical signals, and vascularized 3D skin models with dynamic perfusion and microfluidic devices known as skin on a chip. Finally, ex vivo skin models are presented that could more accurately represent human skin in terms of structure, cell signaling mechanisms, and absorption effects. Although the current development of models without the use of animals is promising, improvements and refinements are needed to make the models more suitable as screening platforms for topical anti-inflammatory drugs.

Funder

National Technological Institute of Mexico

Publisher

MDPI AG

Subject

Pharmaceutical Science

Reference104 articles.

1. Inflamación;Cienc. Exact. Fís. Nat.,2008

2. Kindt, T.J., Goldsby, R.A., Osborne, B.A., and Kuby, J. (2007). Kuby Immunology, W.H. Freeman. [6th ed.].

3. Improved Corrosion Performance of Biodegradable Magnesium in Simulated Inflammatory Condition via Drug-Loaded Plasma Electrolytic Oxidation Coatings;Yarmand;Mater. Chem. Phys.,2020

4. Surface Functionalization of Anodized Tantalum with Mn3O4 Nanoparticles for Effective Corrosion Protection in Simulated Inflammatory Condition;Bahrampour;Ceram. Int.,2022

5. Systemic Inflammation after Trauma;Lenz;Injury,2007

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3