Neuropharmacological Activity of the Acetonic Extract of Malpighia mexicana A. Juss. and Its Phytochemical Profile
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Published:2023-10-10
Issue:4
Volume:91
Page:47
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ISSN:2218-0532
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Container-title:Scientia Pharmaceutica
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language:en
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Short-container-title:Sci. Pharm.
Author:
Avilés-Montes Dante1, Salinas-Sánchez David Osvaldo23ORCID, Sotelo-Leyva César4ORCID, Zamilpa Alejandro5ORCID, Batalla-Martinez Franceli Itzel3, Abarca-Vargas Rodolfo6ORCID, Rivas-González Juan Manuel3, Dorado Óscar7, Figueroa-Brito Rodolfo8, Petricevich Vera L.6ORCID, Morales-Ferra Dulce Lourdes5ORCID, González-Cortazar Manasés4ORCID
Affiliation:
1. Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico 2. Centro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico 3. Escuela de Estudios Superiores del Jicarero, Universidad Autónoma del Estado de Morelos, Galeana-Tequesquitengo S/N, Col. El Jicarero, Jojutla 62909, Morelos, Mexico 4. Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Chilpancingo 39000, Guerrero, Mexico 5. Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico 6. Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros, Esquina Iztaccíhuatl S/N, Col. Volcanes, Cuernavaca 62350, Morelos, Mexico 7. Centro de Educación Ambiental e Investigación Sierra de Huautla, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico 8. Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla Km 6, AP 24, San Isidro, Yautepec 62731, Morelos, Mexico
Abstract
Mental and neurological disorders are conditions that affect thoughts, emotions, behavior, and relationships. Malpighia mexicana A. Juss. is a plant used in Mexican traditional medicine for the treatment of such disorders. This work aimed to investigate the antidepressant, anxiolytic, sedative, hypnotic, and anticonvulsant effects of the acetonic extract (MmAE) of M. mexicana and its fractions (F3, F4-10, F14) using the forced swimming test (FST), elevated plus maze (EPM), open field test (OFT), pentobarbital-induced sleep test (PBTt), and pentylenetetrazol-induced seizure test (PTZt). MmAE, F3, F4-10, F14, and vehicle were administrated orally 24, 18, and 1 h prior to the evaluations. Imipramine (15 mg/kg, p.o.) was administrated 1 h prior to the evaluations as a positive control for the FST, while diazepam (1 mg/kg, p.o.) was administrated 1 h prior to the evaluations as a positive control for the EPM, OFT, PBTt, and PTZt. MmAE had an anxiolytic effect; MmAE and F3, F4-10, and F14 showed an antidepressant effect, sedative effect, hypnotic effect, and anticonvulsant effect. Using HPLC, we identified the compounds quercetin 3-O-rutinoside (1), kaempferol 3-O-glucoside (2), luteolin 7-O-glucoside (3), quercetin (4), and kaempferol (5) in MmAE and compounds (1), (2), and (3) in F14. Using GC-MS, we identified α-tocopherol, phytol, and β-amyrin in F3; β-tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10; and α- tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10. The neuropharmacological effects found in this work may be due to the presence of vitamins, phytosterols, terpenes, and flavonoids. This research requires further study to clarify the mechanisms of action of the identified compounds.
Subject
Pharmaceutical Science
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