Pineapple Waste Cell Wall Sugar Fermentation by Saccharomyces cerevisiae for Second Generation Bioethanol Production

Abstract

Agricultural food waste is rich in cellulosic and non-cellulosic fermentable substance. In this study, we investigated the bioconversion of pineapple waste cell wall sugars into bioethanol by simultaneous saccharification and fermentation using Saccharomyces cerevisiae ATCC 4126. Soluble and insoluble cell wall sugars were investigated during the fermentation process. Moreover, the fermentation medium was investigated for protein, moisture, ash, lignin and glycerol determinations with a particular focus on the increase in single cell protein due to yeast growth, allowing a total valorization of the resulting fermentation medium, with no further waste production, with respect to environmental sustainability. Soluble and insoluble sugars in the starting material were 32.12% and 26.33% respectively. The main insoluble sugars resulting from the cell wall hydrolysis detected at the beginning of the fermentation, were glucose, xylose and uronic acid. Glucose and mannose were the most prevalent sugars in the soluble sugars fraction. The ethanol theoretical yield, calculated according to dry matter lost, reached up to 85% (3.9% EtOH). The final fermentation substrate was mainly represented by pentose sugars. The protein content increased from 4.45% up to 20.1% during the process.