Generation of Mutants from the 57B Region of Drosophila melanogaster-Reference-Cited by-同舟云学术

Generation of Mutants from the 57B Region of Drosophila melanogaster

Published:2023-11-06 Issue:11 Volume:14 Page:2047
ISSN:2073-4425
Container-title:Genes
language:en
Short-container-title:Genes

Author:

Steinmetz Eva Louise¹²^ORCID,Noh Sandra¹,Klöppel Christine¹,Fuhr Martin F.¹,Bach Nicole¹,Raffael Mona Evelyn¹,Hildebrandt Kirsten¹,Wittling Fabienne¹³,Jann Doris¹⁴,Walldorf Uwe¹

Affiliation:

1. Developmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, Germany

2. Zoology & Physiology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building B2.1, D-66123 Saarbrücken, Germany

3. Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Saarland University, Building E8.1, D-66123 Saarbrücken, Germany

4. Medical Biochemistry & Molecular Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 45.2, D-66421 Homburg, Germany

Abstract

The 57B region of Drosophila melanogaster includes a cluster of the three homeobox genes orthopedia (otp), Drosophila Retinal homeobox (DRx), and homeobrain (hbn). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for otp, DRx, and hbn. With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of Drosophila, which could now be used to further explore the functions of the corresponding genes in the future.

Publisher

MDPI AG

Subject

Genetics (clinical),Genetics

Link

https://www.mdpi.com/2073-4425/14/11/2047/pdf

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