Abstract
The reported association of Moringa oleifera seeds and allergic disease clinically resembling occupational asthma in cosmetic manufacturing workers has resultedin the need to identify such components in the manufacturing process. However, Moringa oleifera leaves from the same plant, an important food ingredient, have limited immunotoxicity data. This study aimed to determine if Moringa oleifera leafproteins (MLP) can elicit allergic responses in BALB/c mice. The BALB/c mice were sensitized twice and challenged 10 times to evaluate the potential allergenicityof MLP in vivo. The results showed increased levels of mast cells, total and specific IgE and IgG, severe signs of systemic anaphylaxis, and reduced body temperature compared with controls. The sensitized mice serum observed enhanced levels of histamine and Th-related cytokine release. Compared with the control group, increased levels of interleukins IL-4, IL-9, and IL-17A and enhanced expression and secretion of normal T cells were found in the culture supernatant of splenocytes treated with MLP.This study suggeststhat MLPcanelicit allergic responses; this providesmore comprehensive guidance for identifying new allergen candidates and developing hypoallergenic MLP products.
Funder
the Research Program of State Key Laboratory of Food Science and Technology, Nanchang University
Central Government Guide Local Special Fund Project for Scientific and Technological Development of Jiangxi Province
Subject
Food Science,Nutrition and Dietetics
Reference68 articles.
1. Occupational asthma to "the miracle tree" (Moringa oleifera): First description;Poussel;J. Allergy Clin. Immunol. Pract.,2015
2. Occupational asthma;Tarlo;N. Engl. J. Med.,2014
3. Oxidative DNA damage protective activity, anti-oxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem. Toxicol;Singh;Int. J. Publ. Br. Ind. Biol. Res. Assoc.,2009
4. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells;Sreelatha;Food Chem. Toxicol. Int. J. Publ. Br. Ind. Biol. Res. Assoc.,2011
5. In vitro/in vivo hepatoprotective properties of 1-O-(4-hydroxymethylphenyl)-alpha-L-rhamnopyranoside from Moringa oleifera seeds against carbon tetrachloride-induced hepatic injury;Sun;Food Chem. Toxicol. Int. J. Publ. Br. Ind. Biol. Res. Assoc.,2019
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献