HLA-G 14 bp Ins/Del (rs66554220) Variant Is Not Associated with Breast Cancer in Women from Western Mexico

Author:

Becerra-Loaiza Denisse Stephania12,Roldan Flores Luisa Fernanda1,Ochoa-Ramírez Luis Antonio3,Gutiérrez-Zepeda Bricia M.12ORCID,Del Toro-Arreola Alicia1,Franco-Topete Ramón Antonio4,Morán-Mendoza Andrés5,Oceguera-Villanueva Antonio6,Topete Antonio1,Javalera David7,Quintero-Ramos Antonio18ORCID,Daneri-Navarro Adrián1

Affiliation:

1. Centro Universitario de Ciencias de la Salud, Laboratorio de Inmunología, Departamento de Fisiología, Universidad de Guadalajara, Sierra Mojada #950, Guadalajara 44340, Mexico

2. Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Departamento de Biología Molecular y Genómica, Universidad de Guadalajara, Sierra Mojada #950, Guadalajara 44340, Mexico

3. Laboratorio de Medicina Genómica, Hospital General de Culiacán, Culiacán 80230, Mexico

4. Centro Universitario de Ciencias de la Salud, Laboratorio de Patología, Departamento de Microbiología y Patología, Universidad de Guadalajara, Sierra Mojada #950, Guadalajara 44340, Mexico

5. Centro Médico Nacional de Occidente, Hospital de Gineco Obstetricia, Instituto Mexicano del Seguro Social, Av. Belisario Domínguez #1000, Guadalajara 44340, Mexico

6. Instituto Jalisciense de Cancerología, Secretaría de Salud, Coronel Calderón #715, Guadalajara 44280, Mexico

7. Departamento de Aparatos y Sistemas II, Universidad Autónoma de Guadalajara, Av. Patria #1201, Zapopan 45129, Mexico

8. Centro Médico Nacional de Occidente, Unidad de Investigación Biomédica 02, Hospital de Especialidades, Instituto Mexicano del Seguro Social, Av. Belisario Domínguez #999, Guadalajara 44340, Mexico

Abstract

HLA-G is a physiology and pathologic immunomodulator detrimentally related to cancer. Its gene is heavily transcriptionally and post-transcriptionally regulated by variants located in regulator regions like 3′UTR, being the most studied Ins/Del of 14-bp (rs66554220), which is known to influence the effects of endogen cell factors; nevertheless, the reports are discrepant and controversial. Herein, the relationship of the 14-bp Ins/Del variant (rs66554220) with breast cancer (BC) and its clinical characteristics were analyzed in 182 women with non-familial BC and 221 disease-free women as a reference group. Both groups from western Mexico and sex–age-matched (sm-RG). The rs66554220 variant was amplified by SSP-PCR and the fragments were visualized in polyacrylamide gel electrophoresis. The variant rs66554220 was not associated with BC in our population. However, we suggest the Ins allele as a possible risk factor for developing BC at clinical stage IV (OR = 3.05, 95% CI = 1.16–7.96, p = 0.01); nevertheless, given the small stratified sample size (n = 11, statistical power = 41%), this is inconclusive. In conclusion, the 14-bp Ins/Del (rs66554220) variant of HLA-G is not associated with BC in the Mexican population, but might be related to advanced breast tumors. Further studies are required.

Funder

Universidad de Guadalajara

Publisher

MDPI AG

Subject

Microbiology (medical),Molecular Biology,General Medicine,Microbiology

Reference40 articles.

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