Culture Shock: An Investigation into the Tolerance of Pathogenic Biofilms to Antiseptics in Environments Resembling the Chronic Wound Milieu

Author:

Paleczny Justyna1ORCID,Brożyna Malwina1,Dudek Bartłomiej1,Woytoń Aleksandra1,Chodaczek Grzegorz2ORCID,Szajnik Marta3,Junka Adam1ORCID

Affiliation:

1. Platform for Unique Models Application, Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, Poland

2. Bioimaging Laboratory, Lukasiewicz Research Network—PORT Polish Center for Technology Development, 54-066 Wroclaw, Poland

3. Faculty of Medicine, Lazarski University, 02-662 Warsaw, Poland

Abstract

Credible assessment methods must be applied to evaluate antiseptics’ in vitro activity reliably. Studies indicate that the medium for biofilm culturing should resemble the conditions present at the site of infection. We cultured S. aureus, S. epidermidis, P. aeruginosa, C. albicans, and E. coli biofilms in IVWM (In Vitro Wound Milieu)—the medium reflecting wound milieu—and were compared to the ones cultured in the laboratory microbiological Mueller–Hinton (MH) medium. We analyzed and compared crucial biofilm characteristics and treated microbes with polyhexamethylene biguanide hydrochloride (PHMB), povidone-iodine (PVP-I), and super-oxidized solution with hypochlorites (SOHs). Biofilm biomass of S. aureus and S. epidermidis was higher in IVWM than in MH medium. Microbes cultured in IVWM exhibited greater metabolic activity and thickness than in MH medium. Biofilm of the majority of microbial species was more resistant to PHMB and PVP-I in the IVWM than in the MH medium. P. aeruginosa displayed a two-fold lower MBEC value of PHMB in the IVWM than in the MH medium. PHMB was more effective in the IVWM than in the MH medium against S. aureus biofilm cultured on a biocellulose carrier (instead of polystyrene). The applied improvement of the standard in vitro methodology allows us to predict the effects of treatment of non-healing wounds with specific antiseptics.

Funder

National Science Centre, Poland

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Quality of life case series review: wound bed preparation from a UK perspective;British Journal of Community Nursing;2024-06-01

2. Recent Approaches to Wound Treatment—Second Edition;International Journal of Molecular Sciences;2024-05-15

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