Polyphenolic Compounds Nanostructurated with Gold Nanoparticles Enhance Wound Repair
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Published:2023-12-05
Issue:24
Volume:24
Page:17138
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Martínez-Cuazitl Adriana12, Gómez-García María del Consuelo1, Pérez-Mora Salvador1ORCID, Rojas-López Marlon3, Delgado-Macuil Raúl Jacobo3, Ocampo-López Juan4ORCID, Vázquez-Zapién Gustavo Jesús5ORCID, Mata-Miranda Mónica Maribel2ORCID, Pérez-Ishiwara David Guillermo1
Affiliation:
1. Laboratorio de Biomedicina Molecular, ENMyH, Instituto Politécnico Nacional, Mexico City 07320, Mexico 2. Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Universidad del Ejército y Fuerza Aérea-Secretaría de la Defensa Nacional, Mexico City 11200, Mexico 3. Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Santa Inés Tecuexcomac 90700, Mexico 4. Laboratorio de Histología e Histopatología del Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo 42090, Mexico 5. Centro de Investigación y Desarrollo del Ejército y Fuerza Aérea Mexicanos de la Secretaría de la Defensa Nacional (CIDEFAM—SEDENA), Mexico City 11400, Mexico
Abstract
Gold nanoparticles (AuNPs) have been used in a wide range of applications, conferring to bio-molecules diverse properties such as delivery, stabilization, and reduction of the adverse effects of drugs or plant extracts. Polyphenolic compounds from Bacopa procumbens (B. procumbens) (BP) can modulate proliferation, adhesion, migration, and cell differentiation, reducing the artificial scratch area in fibroblast cultures and promoting wound healing in an in vivo model. Here, chemically synthesized AuNPs conjugated with BP (AuNP-BP) were characterized using UV-Vis, ATR-FTIR, DLS, zeta-potential, and TEM analysis. The results showed an overlap of the FTIR spectra of the polyphenolic compounds from B. procumbens adhered to the surface of the AuNPs. UV-vis analysis indicated that the average size of the AuNP-BP was 28 nm, while DLS analysis showed a size of 44.58 nm and, by TEM, a size of 16.5 nm with an icosahedral morphology was observed. These measurements suggest an increase in the size of the nanoparticles after conjugation with BP, compared to the sizes of 9 nm, 44.51 nm, and 14.17 nm for the unconjugated AuNPs, respectively. Furthermore, the zeta potential of the AuNPs, which was originally −36.3 ± 12.3 mV shifted to −18.2 ± 7.02 mV after conjugation with BP, indicating improved stability of the nanoparticles. Enhancement of the wound healing effect was evaluated by morphometric, histochemical, and FTIR changes in a rat wound excision model. Results showed that the nanoconjugation process reduced the BP concentrations by 100-fold to have the same wound healing effect as BP alone. Besides, histological and FTIR spectroscopy analyses demonstrated that AuNP-BP treatment exhibited better macroscopical performance, showing a reduction in inflammatory cells and an increased synthesis and improved organization of collagen fibers.
Funder
SIP-IPN innovation-IPN
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference35 articles.
1. Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing;Kwan;Nanomedicine,2011 2. The wound healing process: An overview of the cellular and molecular mechanisms;Velnar;J. Int. Med. Res.,2009 3. Spampinato, S.F., Caruso, G.I., De Pasquale, R., Sortino, M.A., and Merlo, S. (2020). The Treatment of Impaired Wound Healing in Diabetes: Looking among Old Drugs. Pharmaceuticals, 13. 4. Naskar, A., and Kim, K.S. (2020). Recent Advances in Nanomaterial-Based Wound-Healing Therapeutics. Pharmaceutics, 12. 5. Wound Healing and the Use of Medicinal Plants;Shedoeva;Evid. Based Complement. Altern. Med.,2019
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