Affiliation:
1. Central Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, Russia
Abstract
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a unique genome editing tool that can be easily used in a wide range of applications, including functional genomics, transcriptomics, epigenetics, biotechnology, plant engineering, livestock breeding, gene therapy, diagnostics, and so on. This review is focused on the current CRISPR/Cas9 landscape, e.g., on Cas9 variants with improved properties, on Cas9-derived and fusion proteins, on Cas9 delivery methods, on pre-existing immunity against CRISPR/Cas9 proteins, anti-CRISPR proteins, and their possible roles in CRISPR/Cas9 function improvement. Moreover, this review presents a detailed outline of CRISPR/Cas9-based diagnostics and therapeutic approaches. Finally, the review addresses the future expansion of genome editors’ toolbox with Cas9 orthologs and other CRISPR/Cas proteins.
Funder
Ministry of Science and Higher Education of the Russian Federation
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference381 articles.
1. Gaj, T., Sirk, S.J., Shui, S.-L., and Liu, J. (2016). Genome-Editing Technologies: Principles and Applications. Cold Spring Harb. Perspect. Biol., 8.
2. Applications of Genome Editing Technology in the Targeted Therapy of Human Diseases: Mechanisms, Advances and Prospects;Li;Signal Transduct. Target. Ther.,2020
3. The History of Genome Editing: Advances from the Interface of Chemistry & Biology;Matsumoto;Chem. Commun.,2023
4. High-Content CRISPR Screening;Bock;Nat. Rev. Methods Primers,2022
5. Grav, L.M., la Cour Karottki, K.J., Lee, J.S., and Kildegaard, H.F. (2017). Methods in Molecular Biology, Springer.