A Berberine-Loaded Bletilla striata Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing
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Published:2023-11-14
Issue:22
Volume:24
Page:16286
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Hu Zhengbo1, Zhao Kai1, Chen Xingcan1, Zhou Mingyuan1, Chen Yuchi1, Ye Xiaoqing1, Zhou Fangmei1, Ding Zhishan1, Zhu Bingqi1
Affiliation:
1. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China
Abstract
The healing process of a diabetic wound (DW) is often impeded by a series of interrelated factors, including severe infection, persistent inflammation, and excessive oxidative stress. Therefore, it is particularly crucial to develop a medical dressing that can address these issues simultaneously. To this end, different ratios of Bletilla striata polysaccharide (BSP) and berberine (BER) were physically blended with Carbomer 940 (CBM940) to develop a composite hydrogel as a medical dressing. The BSP/BER hydrogel was characterized using SEM, FTIR, rheological testing and other techniques. The anti-inflammatory, antioxidant, and antibacterial properties of the hydrogel were evaluated using cell and bacterial models in vitro. A DW model of ICR mice was established to evaluate the effect of the hydrogel on DW healing in vivo. The hydrogel exhibited excellent biocompatibility and remarkable antibacterial, anti-inflammatory, and antioxidant properties. In addition, animal experiments showed that the BSP/BER hydrogel significantly accelerated wound healing in DW mice. Among the different formulations, the LBSP/BER hydrogel (2% BSP, mBER:mBSP = 1:40) demonstrated the most remarkable efficacy. In conclusion, the BSP/BER hydrogel developed exhibited immense properties and great potential as a medical dressing for the repair of DW, addressing a crucial need in clinical practice.
Funder
Zhejiang Provincial Public Welfare Research Project Zhejiang Medical Science and Technology Program Zhejiang Traditional Chinese Medicine Scientific Research Program
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference48 articles.
1. Ferroptosis: Roles and molecular mechanisms in diabetic cardiomyopathy;Zhao;Front. Endocrinol.,2023 2. Tan, C.T., Liang, K., Ngo, Z.H., Dube, C.T., and Lim, C.Y. (2020). Application of 3D Bioprinting Technologies to the Management and Treatment of Diabetic Foot Ulcers. Biomedicines, 8. 3. Wang, Y., Pi, Y., Hu, L., Peng, Z., Hu, H., Zhao, J., Zhou, Y., and Wang, D. (2023). Proteomic analysis of foot ulcer tissue reveals novel potential therapeutic targets of wound healing in diabetic foot ulcers. Comput. Biol. Med., 159. 4. Etiology, Epidemiology, and Disparities in the Burden of Diabetic Foot Ulcers;McDermott;Diabetes Care,2023 5. A meta-analysis of mortality after minor amputation among patients with diabetes and/or peripheral vascular disease;Yammine;J. Vasc. Surg.,2020
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