Affiliation:
1. Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Street, 87-100 Toruń, Poland
Abstract
The main goal of this work was to develop analytical procedures for the isolation and determination of selected isothiocyanates. As an example, particularly sulforaphane from plants of the Brassicaceae Burnett or Cruciferae Juss family. The applied methodology was mainly based on classical extraction methods and high-performance liquid chromatography coupled with tandem mass spectrometry. Moreover, the effect of temperature on the release of isothiocyanates from plant cells was considered. The cytotoxic activity of the obtained plant extracts against a selected cancer cell line has also been included. The results allow evaluating the usefulness of obtained plant extracts and raw sprouts regarding their content of isothiocyanates—bioactive compounds with chemopreventive properties.
Funder
National Center for Re-search and Development
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference54 articles.
1. Bajkacz, S., Ligor, M., Baranowska, I., and Buszewski, B. (2021). Separation and determination of chemopreventive phytochemicals of flavonoids from Brassicaceae plants. Molecules, 26.
2. Brassicaceae—A classical review on its pharmacological activities;Shankar;Int. J. Pharm. Sci. Rev. Res.,2019
3. Health promoting effects of phytochemicals from brassicaceae: A review;Mann;Indian J. Pharm. Biol. Res.,2013
4. Raiola, A., Errico, A., Petruk, G., Monti, M.D., Barone, A., and Rigano, M.M. (2018). Bioactive compounds in brassicaceae vegetables with a role in the prevention of chronic diseases. Molecules, 23.
5. 7-Methylsulfinylheptyl and 8-methylsulfinyloctyl isothiocyanates from watercress are potent inducers of phase II enzymes;Rose;Carcinogenesis,2000