Affiliation:
1. Department of Pharmacy, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, Italy
2. International Institution of Heliciculture of Cherasco-Lumacheria Italiana s.r.l., 12062 Cherasco, Italy
Abstract
Snail slime (SS) is a viscous secretion obtained from different snail species. SS composition is variable according to factors such as the extraction method. Even if several papers have been published regarding this topic, the molecular mechanisms at the base of SS biological effects remain unexplored. Thus, the aim of this study is to evaluate the capability of SS, extracted with the cruelty-free Muller method, to promote viability and angiogenesis processes and, in parallel, to counteract inflammation occurrence on skin cell populations. SS was administered to keratinocytes, macrophages and fibroblasts, then cell viability, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, cytotoxicity by lactate dehydrogenase (LDH) assay, morphology by haematoxylin-eosin staining, gene and protein expression through real-time polymerase chain reaction (PCR) and Western blot, cell cycle phases by flow cytometry, and collagen secretion using an enzyme-linked immunosorbent assay (ELISA) test, were measured. Our results evidence SS capability to promote fibroblast viability and to trigger recovery mechanisms by activating the Erk protein. Moreover, an appreciable anti-inflammatory effect due to the significant reduction in cyclooxygenase-2 expression, and a positive modulation of new blood vessel formation demonstrated by increased Angiopoietin 1 gene expression and a higher matrix deposition (evidenced by the augmented amount of released collagen I) can be identified. This evidence led us to assume that the Muller method extracted-SS represents a valuable and promising natural product suitable for cosmetic and skin care formulations.
Funder
University “G. d’Annunzio” Chieti-Pescara
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference30 articles.
1. Snail mucus–glandular origin and composition in Helix pomatia;Greistorfer;Zoology,2017
2. Helix and Drugs: Snails for Western Health Care From Antiquity to the Present;Bonnemain;Evid. Based Complement. Alternat. Med.,2005
3. L’effect bronchorelaxant de l’Hélicidine, un extrait d’Helix pomatia, fait intervenir une libération de prostaglandine E2 [The bronchorelaxant effect of helicidine, a Helix pomatia extract, interferes with prostaglandin E2];Pons;Pathol. Biol.,1999
4. Novel drug-loaded film forming patch based on gelatin and snail slime;Albertini;Int. J. Pharm.,2021
5. Cristiano, L., and Guagni, M. (2022). Zooceuticals and Cosmetic Ingredients Derived from Animals. Cosmetics, 9.
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