Evaluation of the Cytotoxic, Antioxidative and Antimicrobial Effects of Dracocephalum moldavica L. Cultivars
-
Published:2023-02-07
Issue:4
Volume:28
Page:1604
-
ISSN:1420-3049
-
Container-title:Molecules
-
language:en
-
Short-container-title:Molecules
Author:
Simea Ștefania1, Ielciu Irina2, Hanganu Daniela3, Niculae Mihaela4ORCID, Pall Emoke4ORCID, Burtescu Ramona Flavia5, Olah Neli-Kinga56, Cenariu Mihai4, Oniga Ilioara3ORCID, Benedec Daniela3ORCID, Duda Marcel1ORCID
Affiliation:
1. Department of Crop Science, Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania 2. Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, 400010 Cluj-Napoca, Romania 3. Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, 400000 Cluj-Napoca, Romania 4. Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400374 Cluj-Napoca, Romania 5. SC PlantExtrakt SRL, 407059 Rădaia, Cluj-Napoca, Romania 6. Department of Therapeutical Chemistry, Pharmaceutical Industry and Biotechnologies, Faculty of Pharmacy, “Vasile Goldiș” Western University from Arad, 310048 Arad, Romania
Abstract
The aim of the present study was to correlate the antioxidant, antimicrobial, and cytotoxic activities of hydroalcoholic extracts obtained from the aerial parts of three Dracocephalum moldavica L. cultivars with their polyphenolic compositions. The polyphenols were identified and quantified using spectrophotometrical methods and LC–MS analysis. Their antioxidant capacities were assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Their in vitro antimicrobial efficacies were assessed using the agar well diffusion and broth microdilution methods. Their cytotoxicity was investigated on normal diploid foreskin fibroblasts (BJ) and on colorectal adenocarcinoma (DLD-1) cell lines. The results pointed out significant amounts of polyphenolic compounds in the compositions of the tested cultivars, with rosmarinic acid as the main compound (amounts ranging between 5.337 ± 0.0411 and 6.320 ± 0.0535 mg/mL). All three cultivars displayed significant antioxidant (IC50 ranging between 35.542 ± 0.043 and 40.901 ± 0.161 µg/mL for the DPPH assay, and for the FRAP assay 293.194 ± 0.213 and 330.165 ± 0.754 µmol Trolox equivalent/mg dry vegetal material) and antimicrobial potential (especially towards the Gram-positive bacteria), as well as a selective toxicity towards the tumoral line. A significant positive correlation was found between antioxidant activity and the total phenolic acids (r2 = 0.987) and polyphenols (r2 = 0.951). These findings bring further arguments for strongly considering D. moldavica cultivars as promising vegetal products, which warrants further investigation.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference75 articles.
1. Aćimović, M., Šovljanski, O., Šeregelj, V., Pezo, L., Zheljazkov, V.D., Ljujić, J., Tomić, A., Ćetković, G., Čanadanović-Brunet, J., and Miljković, A. (2022). Chemical Composition, Antioxidant, and Antimicrobial Activity of Dracocephalum Moldavica L. Essential Oil and Hydrolate. Plants, 11. 2. Changes in Content and Chemical Composition of Dracocephalum Moldavica L. Essential Oil at Different Harvest Dates;Sabra;J. Med. Plants Stud.,2015 3. Moldovan, C., Nițu, S., Hermeziu, M., Vidican, R., Sandor, M., Gâdea, Ș., David, A., Stoian, V.A., Vâtcă, S.D., and Stoian, V. (2022). Growth Characteristics of Dracocephalum Moldavica L. in Relation to Density for Sustainable Cropping Technology Development. Agriculture, 12. 4. Sites of Secretion of Bioactive Compounds in Leaves of Dracocephalum Moldavica L.: Anatomical, Histochemical, and Essential Oil Study;Dmitruk;Rev. Bras. Bot.,2019 5. Evaluation of Common Bean (Phaseolus Vulgaris L.) Seed Yield and Quali-Quantitative Production of the Essential Oils from Fennel (Foeniculum Vulgare Mill.) and Dragonhead (Dracocephalum Moldavica L.) in Intercropping System under Humic Acid Application;Javanmard;J. Clean. Prod.,2019
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|