Phloretin Inhibits Quorum Sensing and Biofilm Formation in Serratia marcescens

Author:

Qi Yueheng12,Ji Pengcheng3,Yin Kunyuan3,Zheng Yi3,Niu Jiangxiu2,Jia Aiqun1,Zhou Jinwei3,Li Jingguo1ORCID

Affiliation:

1. Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou 477150, China

2. Luoyang Key Laboratory of Organic Functional Molecules, College of Food and Drug, Luoyang Normal University, Luoyang 471934, China

3. School of Food and Biological Engineering, Xuzhou University of Technology, Xuzhou 221018, China

Abstract

This study investigated the antivirulence capacity and mechanism of apple-skin-derived phloretin against Serratia marcescens NJ01, a vegetable spoilage bacterium. At 0.5 to 2 mg/mL doses, phloretin considerably inhibited the secretion of acyl homoserine lactones (AHLs), indicating that phloretin disrupted quorum sensing (QS) in S. marcescens NJ01. The dysfunction of QS resulted in reduced biofilms and the decreased production of protease, prodigiosin, extracellular polysaccharides (EPSs), and swimming and swarming motilities. Dysfunctional QS also weakened the activity of antioxidant enzymes and improved oxidative injury. The improved oxidative injury changed the composition of the membrane, improved membrane permeability, and eventually increased the susceptibility of biofilm cells to amikacin, netilmicin, and imipenem. The disrupted QS and enhanced oxidative stress also caused disorders of amino acid metabolism, energy metabolism, and nucleic acid metabolism, and ultimately attenuated the ability of S. marcescens NJ01 to induce spoilage. Our results indicated that phloretin can act as a potent drug to defend against spoilage by S. marcescens.

Funder

National Natural Science Foundation of China

Jiangsu Youth Talent Promotion Project

Major Natural Science Research Project in Colleges and Universities of Jiangsu Province

Henan Province Science and Technology Attack Plan Foundation

Key Scientific Research Project of Higher Education of Henan Province

Key Scientific and Technological Project of Henan Province of China

innovative training program for college students in Henan Province of China

Publisher

MDPI AG

Subject

Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science

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