Phenolic Compound, Antioxidant, Antibacterial, and In Silico Studies of Extracts from the Aerial Parts of Lactuca saligna L.
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Published:2024-01-25
Issue:3
Volume:29
Page:596
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Bouymajane Aziz12, Filali Fouzia Rhazi2, Moujane Soumia3, Majdoub Yassine Oulad El4, Otzen Philipp5ORCID, Channaoui Souhail6ORCID, Ed-Dra Abdelaziz7ORCID, Bouddine Toufik8, Sellam Khalid1, Boughrous Ali Ait1, Miceli Natalizia4ORCID, Altemimi Ammar B.9ORCID, Cacciola Francesco10ORCID
Affiliation:
1. Biology, Environment and Health Team, Faculty of Sciences and Technologies, Moulay Ismail University, Meknes 50070, Morocco 2. Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, Meknes 50070, Morocco 3. Biochemistry of Natural Substances, Faculty of Science and Techniques, Moulay Ismail University, Errachdia 50003, Morocco 4. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy 5. Institute of Anorganic and Analytical Chemistry, University of Münster, Corrensstraße 48, 48149 Münster, Germany 6. Oasis System Research Unit, Regional Center of Agricultural Research of Errachidia, National Institute of Agricultural Research, P.O. Box 415, Rabat 10090, Morocco 7. Laboratory of Engineering and Applied Technologies, Higher School of Technology, M’ghila Campus, Sultan Moulay Slimane University, Beni Mellal 23000, Morocco 8. Bioactive Molecules, Health and Biotechnology, Centre of Technology and Transformation, Faculty of Sciences, Moulay Ismail University, Meknes 50070, Morocco 9. Food Science Department, College of Agriculture, University of Basrah, Basrah 61004, Iraq 10. Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy
Abstract
Medicinal plants are considered a major source for discovering novel effective drugs. To our knowledge, no studies have reported the chemical composition and biological activities of Moroccan Lactuca saligna extracts. In this context, this study aims to characterize the polyphenolic compounds distributed in hydro-methanolic extracts of L. saligna and evaluate their antioxidant and antibacterial activities; in addition, in silico analysis based on molecular docking and ADMET was performed to predict the antibacterial activity of the identified phenolic compounds. Our results showed the identification of 29 among 30 detected phenolic compounds with an abundance of dicaffeoyltartaric acid, luteolin 7-glucoronide, 3,5-di-O-caffeoylquinic acid, and 5-caffeoylquinic acid with 472.77, 224.30, 196.79, and 171.74 mg/kg of dried extract, respectively. Additionally, antioxidant activity assessed by DPPH scavenging activity, ferric reducing antioxidant power (FRAP) assay, and ferrous ion-chelating (FIC) assay showed interesting antioxidant activity. Moreover, the results showed remarkable antibacterial activity against Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, and Listeria monocytogenes with minimum inhibitory concentrations between 1.30 ± 0.31 and 10.41 ± 0.23 mg/mL. Furthermore, in silico analysis identified three compounds, including Apigenin 7-O-glucuronide, Quercetin-3-O-glucuronide, and 3-p-Coumaroylquinic acid as potent candidates for developing new antibacterial agents with acceptable pharmacokinetic properties. Hence, L. saligna can be considered a source of phytochemical compounds with remarkable activities, while further in vitro and in vivo studies are required to explore the main biological activities of this plant.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
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