miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study
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Published:2024-02-06
Issue:4
Volume:29
Page:748
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Baraldo Nada1ORCID, Buzzoni Luna2, Pasti Luisa3ORCID, Cavazzini Alberto14, Marchetti Nicola1ORCID, Mancia Annalaura25ORCID
Affiliation:
1. Department of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, Italy 2. Department of Life Science and Biotechnologies, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy 3. Department of Environmental and Prevention Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy 4. Council for Agricultural Research and Economics, via della Navicella 2/4, 00184 Rome, Italy 5. Department of Biology and Marine Science, Marine Science Research Institute, 2800 University Blvd N, Jacksonville, FL 32211, USA
Abstract
In this study, the expression and abundance of two candidate chicken (Gallus gallus; gga) microRNAs (miRNAs, miR), gga-miR-21-5p (miR-21) and gga-miR-126-5p (miR-126), have been analyzed in order to identify biomarkers for the traceability and quality of poultry meat. Two breeds of broiler chickens were tested: the most common Ross308 (fast-growing) and the high-quality Ranger Gold (slow-growing). A preliminary analysis of the two miRNAs expressions was conducted across various tissues (liver, lung, spleen, skeletal muscle, and kidney), and the three tissues (lung, spleen, and muscle) with a higher expression were chosen for further analysis. Using quantitative reverse transcription polymerase chain reaction (RT-qPCR), the expression of miRNAs in the three tissues of a total of thirteen animals was determined. The results indicate that miR-126 could be a promising biomarker for the lung tissue in the Ranger Gold (RG) breed (p < 0.01), thus suggesting a potential applicability for tracing hybrids. RG exhibits a significantly higher miR-126 expression in the lung tissue compared to the Ross308 broilers (R308), an indication of greater respiratory capacity and, consequently, a higher oxidative metabolism of the fast-growing hybrid. During sampling, two R308 broilers presented some anomalies, including airsacculitis, hepatic steatosis, and enlarged spleen. The expression of miR-126 and miR-21 was compared in healthy animals and in those presenting anomalies. Chickens with airsacculitis and hepatic steatosis showed an up-regulation of miR-21 and miR-126 in the most commercially valuable tissue, the skeletal muscle or breast (p < 0.05).
Funder
University of Ferrara and the Chamber of Commerce, Industry, Agriculture and Artisanship for the Province of Ferrara
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference60 articles.
1. Meat Quality in Fast-Growing Broiler Chickens;Petracci;World’s Poult. Sci. J.,2015 2. UNAITALIA (2023, December 09). Global and National Meat Consumption Data. Available online: https://www.unaitalia.com/filiera-avicola/. 3. Whitton, C., Bogueva, D., Marinova, D., and Phillips, C.J.C. (2021). Are We Approaching Peak Meat Consumption? Analysis of Meat Consumption from 2000 to 2019 in 35 Countries and Its Relationship to Gross Domestic Product. Animals, 11. 4. Growth, Livability, and Feed Conversion of 1957 versus 2001 Broilers When Fed Representative 1957 and 2001 Broiler Diets;Havenstein;Poult. Sci.,2003 5. (2024, January 20). Welfare Quality R © Consortium, Lelystad N. Welfare Quality Protocols. Available online: http://www.welfarequalitynetwork.net/en-us/reports/assessment-protocols/.
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