Comparative Genomics of Marine Bacteria from a Historically Defined Plastic Biodegradation Consortium with the Capacity to Biodegrade Polyhydroxyalkanoates

Author:

de Vogel Fons A.ORCID,Schlundt Cathleen,Stote Robert E.,Ratto Jo Ann,Amaral-Zettler Linda A.ORCID

Abstract

Biodegradable and compostable plastics are getting more attention as the environmental impacts of fossil-fuel-based plastics are revealed. Microbes can consume these plastics and biodegrade them within weeks to months under the proper conditions. The biobased polyhydroxyalkanoate (PHA) polymer family is an attractive alternative due to its physicochemical properties and biodegradability in soil, aquatic, and composting environments. Standard test methods are available for biodegradation that employ either natural inocula or defined communities, the latter being preferred for standardization and comparability. The original marine biodegradation standard test method ASTM D6691 employed such a defined consortium for testing PHA biodegradation. However, the taxonomic composition and metabolic potential of this consortium have never been confirmed using DNA sequencing technologies. To this end, we revived available members of this consortium and determined their phylogenetic placement, genomic sequence content, and metabolic potential. The revived members belonged to the Bacillaceae, Rhodobacteraceae, and Vibrionaceae families. Using a comparative genomics approach, we found all the necessary enzymes for both PHA production and utilization in most of the members. In a clearing-zone assay, three isolates also showed extracellular depolymerase activity. However, we did not find classical PHA depolymerases, but identified two potentially new extracellular depolymerases that resemble triacylglycerol lipases.

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference127 articles.

1. Plastic waste inputs from land into the ocean

2. Global ecological, social and economic impacts of marine plastic

3. A Pre- and Post-MARPOL Annex V Summary of Hawaiian Monk Seal Entanglements and Marine Debris Accumulation in the Northwestern Hawaiian Islands, 1982–1998

4. Transforming Our World: The 2030 Agenda for Sustainable Developmenthttps://sdgs.un.org/2030agenda

5. Plastic Waste: A European Strategy to Protect the Planet, Defend Our Citizens and Empower Our Industries,2018

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