Hyphal Fusions Enable Efficient Nutrient Distribution in Colletotrichum graminicola Conidiation and Symptom Development on Maize

Abstract

Hyphal and germling fusion is a common phenomenon in ascomycetous fungi. Due to the formed hyphal network, this process enables a coordinated development as well as an interaction with plant hosts and efficient nutrient distribution. Recently, our laboratory work demonstrated a positive correlation between germling fusion and the formation of penetrating hyphopodia on maize leaves outgoing from Colletotrichum graminicola oval conidia. To investigate the probable interconnectivity of these processes, we generated a deletion mutant in Cgso, in which homologs are essential for cellular fusion in other fungal species. However, hyphopodia development was not affected, indicating that both processes are not directly connected. Instead, we were able to link the cellular fusion defect in ∆Cgso to a decreased formation of asexual fruiting bodies of C. graminicola on the leaves. The monitoring of a fluorescent-labelled autophagy marker, eGFP-CgAtg8, revealed a high autophagy activity in the hyphae surrounding the acervuli. These results support the hypothesis that the efficient nutrient transport of degraded cellular material by hyphal fusions enables proper acervuli maturation and, therefore, symptom development on the leaves.