Lactobacillus Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of Pseudomonas aeruginosa from Cystic Fibrosis Lung
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Published:2023-06-29
Issue:7
Volume:11
Page:1707
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Batoni Giovanna1ORCID, Kaya Esingül1, Catelli Elisa1, Quinti Sabrina2, Botti Matteo2, De Carli Alessandro13ORCID, Bianchi Marta1, Maisetta Giuseppantonio1, Esin Semih1ORCID
Affiliation:
1. Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, Italy 2. Cystic Fibrosis Supporting Service, Azienda USL Toscana Nord-Ovest, 57128 Livorno, Italy 3. Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
Abstract
The field of probiotic applications is rapidly expanding, including their use for the control of respiratory tract infections. Nevertheless, probiotics ability to colonize the lung environment and to compete with pulmonary pathogens is still a poorly investigated research area. In this study, we aimed to evaluate the adhesion ability of a number of commercial probiotic strains to the human lung epithelial cell line A549. Furthermore, we assessed probiotic ability to prevent host cell adhesion of one of the major lung pathogens in cystic fibrosis, Pseudomonas aeruginosa, and to reduce the pathogen-induced inflammatory response of human peripheral blood mononuclear cells (PBMCs) in terms of cytokine release. Lactobacillus acidophilus displayed the highest adhesion ability to A549 cells evaluated as percent of adhered bacteria compared to the inoculum. In agreement with such an observation, L. acidophilus was the most efficient in preventing adhesion to A549 cells of a P. aeruginosa isolate from CF sputum. Three-color fluorescence labeling of A549 cells, P. aeruginosa, and L. acidophilus, and confocal microcopy image analyses revealed a likely exclusion effect played by both live and UV-killed L. acidophilus towards P. aeruginosa. Such results were confirmed by CFU count. When co-cultured with PBMCs, both live and UV-killed L. acidophilus reduced the amount of IL-1β and IL-6 in culture supernatants in a statistically significant manner. Overall, the results obtained point to L. acidophilus as an interesting candidate for further studies for a potential aerogenous administration to control P. aeruginosa infections.
Funder
Italian Cystic Fibrosis research foundation PNRR THE – Tuscany Health Ecosystem; Spoke 7 - Innovating Translational Medicine- Sub-project 5 - Innovative models for management of infections caused by antibiotic-resistant bacteria
Subject
Virology,Microbiology (medical),Microbiology
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