Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines

Author:

Salgado Caroline Roberta Soares1,Silva Aldaleia do Nascimento e1,Arruda Igor Falco2,Millar Patrícia Riddell2,Amendoeira Maria Regina Reis2ORCID,Leon Luciane Almeida Amado1,Teixeira Raffaella Bertoni Cavalcanti3ORCID,de Lima Jorge Tiburcio Barbosa4ORCID,Chalhoub Flávia Löwen Levy5,Bispo de Filippis Ana Maria5,Fonseca Ana Beatriz Monteiro6,de Oliveira Jaqueline Mendes1ORCID,Pinto Marcelo Alves1ORCID,Figueiredo Andreza Soriano1ORCID

Affiliation:

1. Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil

2. Laboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil

3. Clínica de Grandes Animais, Departamento de Medicina Veterinária, Universidade Federal de Viçosa—UFV, Viçosa 36570-900, MG, Brazil

4. Departamento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais—UFMG, Belo Horizonte 31270-901, MG, Brazil

5. Laboratório de Arbovírus e Vírus Hemorrágicos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil

6. Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal Fluminense—UFF, Niterói 24210-346, RJ, Brazil

Abstract

Hepatitis E virus (HEV) infection has been demonstrated in various animal species; those recognized as potential zoonotic reservoirs pose a considerable risk to public health. In Brazil, HEV-3 is the only genotype identified in humans and swine nationwide, in a colony-breeding cynomolgus monkey and, recently, in bovines and capybara. There is no information regarding HEV exposure in the equine population in Brazil. This study aimed to investigate anti-HEV antibodies and viral RNA in serum samples from horses slaughtered for meat export and those bred for sport/reproduction purposes. We used a commercially available ELISA kit modified to detect species-specific anti-HEV, using an anti-horse IgG-peroxidase conjugate and evaluating different cutoff formulas and assay precision. Serum samples (n = 257) were tested for anti-HEV IgG and HEV RNA by nested RT-PCR and RT-qPCR. The overall anti-HEV seroprevalence was 26.5% (68/257) without the detection of HEV RNA. Most municipalities (53.3%) and farms (58.8%) had positive horses. Animals slaughtered for human consumption had higher risk of HEV exposure (45.5%) than those bred for sports or reproduction (6.4%) (p < 0.0001). The statistical analysis revealed sex and breeding system as possible risk-associated factors. The first serological evidence of HEV circulation in Brazilian equines reinforces the need for the surveillance of HEV host expansion in a one-health approach.

Funder

Instituto Oswaldo Cruz (IOC)–Fiocruz

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference52 articles.

1. Smith, D., Drexler, J.F., Meng, X.-J., Norder, H., Okamoto, H., van der Poel, W.H.M., Purdy, M.A., Reuter, G., de Souza, W.M., and Ulrich, R.G. (2023, June 05). Taxon Details|ICTV. Available online: https://ictv.global/taxonomy/taxondetails?taxnode_id=202203665.

2. Hepatitis E virus and related viruses in wild, domestic and zoo animals: A review;Spahr;Zoonoses Public Health,2018

3. Hepatitis E virus: Host tropism and zoonotic infection;Wang;Curr. Opin. Microbiol.,2021

4. Kenney, S.P. (2019). The current host range of hepatitis E viruses. Viruses, 11.

5. Analysis of the full-length genome of a Hepatitis E Virus isolate obtained from a wild boar in Japan that is classifiable into a Novel Genotype;Takahashi;J. Gen. Virol.,2011

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3