Caffeic Acid Has Antiviral Activity against Ilhéus Virus In Vitro-Reference-Cited by-同舟云学术

Caffeic Acid Has Antiviral Activity against Ilhéus Virus In Vitro

Published:2023-02-10 Issue:2 Volume:15 Page:494
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Saivish Marielena Vogel¹²^ORCID,Pacca Carolina Colombelli¹³⁴,da Costa Vivaldo Gomes³^ORCID,de Lima Menezes Gabriela⁵⁶^ORCID,da Silva Roosevelt Alves⁶^ORCID,Nebo Liliane⁶,da Silva Gislaine Celestino Dutra¹,de Aguiar Milhim Bruno Henrique Gonçalves¹^ORCID,da Silva Teixeira Igor¹^ORCID,Henrique Tiago⁷,Mistrão Natalia Franco Bueno¹,Hernandes Victor Miranda¹,Zini Nathalia¹,de Carvalho Ana Carolina²^ORCID,Fontoura Marina Alves²^ORCID,Rahal Paula³,Sacchetto Lívia¹^ORCID,Marques Rafael Elias²^ORCID,Nogueira Maurício Lacerda¹²⁸^ORCID

Affiliation:

1. Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, SP, Brazil

2. Brazilian Biosciences National Laboratory, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas 13083-100, SP, Brazil

3. Laboratório de Estudos Genômicos, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto 15054-000, SP, Brazil

4. Faceres Medical School, São José do Rio Preto 15090-000, SP, Brazil

5. Departamento de Biofísica e Farmacologia, Universidade Federal do Rio Grande do Norte, Natal 59072-970, RN, Brazil

6. Unidade Especial de Ciências Exatas, Universidade Federal de Jataí, Jataí 75801-615, GO, Brazil

7. Laboratório de Marcadores Moleculares e Bioinformática, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, SP, Brazil

8. Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555, USA

Abstract

Ilhéus virus (ILHV) is a neglected mosquito-borne flavivirus. ILHV infection may lead to Ilhéus fever, an emerging febrile disease like dengue fever with the potential to evolve into a severe neurological disease characterized by meningoencephalitis; no specific treatments are available for this disease. This study assessed the antiviral properties of caffeic acid, an abundant component of plant-based food products that is also compatible with the socioeconomic limitations associated with this neglected infectious disease. The in vitro activity of caffeic acid on ILHV replication was investigated in Vero and A549 cell lines using plaque assays, quantitative RT-PCR, and immunofluorescence assays. We observed that 500 µM caffeic acid was virucidal against ILHV. Molecular docking indicated that caffeic acid might interact with an allosteric binding site on the envelope protein.

Funder

FAPESP

National Institutes of Health

FAPESP PhD Scholarship

CAPES PhD Scholarship

CNPq PhD Scholarship

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/15/2/494/pdf